Separation of Submicron Bioparticles by Dielectrophoresis
TLDR
With electrode arrays fabricated using direct write electron beam lithography, it is shown that different types of submicron latex spheres can be spatially separated and it is demonstrated that tobacco mosaic virus and herpes simplex virus can be manipulated and spatially separation in a microelectrode array.About:
This article is published in Biophysical Journal.The article was published on 1999-07-01 and is currently open access. It has received 533 citations till now. The article focuses on the topics: Dielectrophoresis & Particle.read more
Citations
More filters
Journal ArticleDOI
Endothelial and stem cell interactions on dielectrophoretically aligned fibrous silk fibroin-chitosan scaffolds
Vishal Gupta,Greg Davis,Alexander Gordon,Andrew M. Altman,Greg P Reece,Peter R. C. Gascoyne,Anshu B. Mathur +6 more
TL;DR: It is concluded that the guidance of cells for regenerative tissue engineering using SFCS scaffolds requires a fine balance between cell-cell interactions that affect the migration speed of cells and the surface characteristics that affects the overall adhesion and direction of migration.
Journal ArticleDOI
Nanoscale dielectrophoretic spectroscopy of individual immobilized mammalian blood cells.
TL;DR: The studies demonstrate the feasibility of using DEPFM to monitor localized changes in membrane capacitance in real time with high spatial resolution on immobilized cells, complementing previous studies of mobile whole cells and cell suspensions.
Journal ArticleDOI
On the Influence of Ion Excluded Volume (Steric) Effects on the Double-Layer Polarization of a Nonconducting Spherical Particle in an AC Field
TL;DR: In this paper, the dipole moment of a charged, dielectric, spherical particle under the influence of a uniform alternating electric field is computed by solving the modified Poisson−Nernst−Planck (PNP) equations (Bikerman's mean-field model) (Philos. Mag. 1942, 33, 384−397) accounting for excluded volume effects of the finite ion size as a function of the double-layer thickness, the electric field frequency, the particle's surface charge, and the volume fraction of the ions in the bulk characterizing the excluded
Journal ArticleDOI
Analytical solutions and validation of electric field and dielectrophoretic force in a bio-microfluidic channel
TL;DR: This paper describes the approach attaining the analytical solution of the dielectrophoretic force expression within a microchannel with parallel facing same size electrodes present on the two faces of channel substrates, with opposite voltages on the pair electrodes.
Journal ArticleDOI
Dielectrically Addressable Microspheres Engineered Using Self-Assembled Monolayers.
TL;DR: These proof of principle studies are the first to demonstrate that the dielectric properties of particles can be specifically engineered to allow their dielectrophoretic manipulation and are a first step toward the development of bead-based dielectophoretic microsystems for multiplexed molecular separation and analysis.
References
More filters
Book
CRC Handbook of Chemistry and Physics
TL;DR: CRC handbook of chemistry and physics, CRC Handbook of Chemistry and Physics, CRC handbook as discussed by the authors, CRC Handbook for Chemistry and Physiology, CRC Handbook for Physics,
Book
Electromechanics of Particles
TL;DR: In this paper, a detailed account of the electromechanical interactions that govern the behaviour of small particles when an electric or magnetic field is present is presented, with numerous real-world examples.
Journal ArticleDOI
Ac electrokinetics: a review of forces in microelectrode structures
TL;DR: In this article, the frequency dependency and magnitude of electrothermally induced fluid flow are discussed for low frequencies (up to 500 kHz) and the effects of Brownian motion, diffusion and buoyancy force are discussed in the context of the controlled manipulation of sub-micrometre particles.
Journal ArticleDOI
The Motion and Precipitation of Suspensoids in Divergent Electric Fields
TL;DR: In this article, it was shown that the phenomena of dielectrophoresis and dielectro-precipitation can be observed under rather ideal conditions, though it is ordinarily often difficult to do so because of the presence of the more easily produced electrophoreis or ion-type migration of charged particles.
Journal ArticleDOI
Separation of Human Breast Cancer Cells From Blood by Differential Dielectric Affinity
Frederick F. Becker,Xiao-Bo Wang,Ying Huang,Ronald Pethig,Jody Vykoukal,Peter R. C. Gascoyne +5 more
TL;DR: Findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.