Journal ArticleDOI
Sequence-Specific Label-Free DNA Sensors Based on Silicon Nanowires
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TLDR
Highly sensitive and sequence-specific DNA sensors were fabricated based on silicon nanowires with single stranded probe DNA molecules covalently immobilized on the nanowire surfaces, recognizing label-free complementary ss-DNA in sample solutions when the target DNA was hybridized with the probe DNA attached on the SiNW surfaces.Abstract:
Highly sensitive and sequence-specific DNA sensors were fabricated based on silicon nanowires (SiNWs) with single stranded (ss) probe DNA molecules covalently immobilized on the nanowire surfaces. Label-free complementary (target) ss-DNA in sample solutions were recognized when the target DNA was hybridized with the probe DNA attached on the SiNW surfaces, producing a change of the conductance of the SiNWs. For a 12-mer oligonucletide probe, 25 pM of target DNA in solution was detected easily (signal/noise ratio > 6), whereas 12-mers with one base mismatch did not produce a signal above the background noise.read more
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Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications
Eugenii Katz,Itamar Willner +1 more
TL;DR: This review describes recent advances in the synthesis of biomolecule-nanoparticle/nanorod hybrid systems and the application of such assemblies in the generation of 2D and 3D ordered structures in solutions and on surfaces.
Journal ArticleDOI
Transport phenomena in nanofluidics
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Journal ArticleDOI
Label-free immunodetection with CMOS-compatible semiconducting nanowires
Eric Stern,James F. Klemic,David A. Routenberg,Pauline N. Wyrembak,Daniel B. Turner-Evans,Andrew D. Hamilton,David A. LaVan,Tarek M. Fahmy,Mark A. Reed +8 more
TL;DR: This work reports an approach that uses complementary metal oxide semiconductor (CMOS) field effect transistor compatible technology and hence demonstrates the specific label-free detection of below 100 femtomolar concentrations of antibodies as well as real-time monitoring of the cellular immune response.
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DNA biosensors and microarrays.
TL;DR: This exhibition celebrates the centenary of the establishment of the University of Lyon with a celebration of the 100th anniversary of the birth of Jean-Bertrand Aristide Dejerine.
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Methods and apparatus for measuring analytes using large scale fet arrays
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References
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Journal ArticleDOI
Nanotube molecular wires as chemical sensors
Jing Kong,Nathan R. Franklin,Chongwu Zhou,Michael Chapline,Shu Peng,Kyeongjae Cho,Hongjie Dai +6 more
TL;DR: The nanotubes sensors exhibit a fast response and a substantially higher sensitivity than that of existing solid-state sensors at room temperature and the mechanisms of molecular sensing with nanotube molecular wires are investigated.
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Enzyme-Coated Carbon Nanotubes as Single-Molecule Biosensors
TL;DR: This first demonstration of nanotube-based biosensors provides a new tool for enzymatic studies and opens the way to biomolecular diagnostics.
Journal ArticleDOI
Photochemical Sensing of NO2 with SnO2 Nanoribbon Nanosensors at Room Temperature
TL;DR: The development of photochemical NO2 sensors that work at room temperature and are based on individual single-crystalline SnO2 nanoribbons are reported, which once again demonstrate that seemingly small variations in ligand structure can result in significant improvements in catalysis.
Journal ArticleDOI
Versatile derivatisation of solid support media for covalent bonding on DNA-microchips
Markus Beier,Jörg D. Hoheisel +1 more
TL;DR: A chemistry was developed that permits on DNA-arrays both the covalent immobilisation of pre-fabricated nucleic acids-such as oligonucleotides, PCR-products or peptide nucleic acid oligomers-and the in situ synthesis of such compounds on either glass or polypropylene surfaces.
Journal ArticleDOI
Immobilization of acrylamide-modified oligonucleotides by co-polymerization
TL;DR: A flexible chemistry for solid phase attachment of oligonucleotides is described, specific for the terminal acrylamide group, and stable probe-containing layers are easily fabricated on supports bearing exposed acrylic groups, including plastic microtiter plates and silanized glass.