Journal ArticleDOI
Solid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acids
Gregg B. Fields,Richard L. Noble +1 more
TLDR
The great variety of conditions under which Fmoc solid phase peptide synthesis may be carried out represents a truly "orthogonal" scheme, and thus offers many unique opportunities for bioorganic chemistry.Abstract:
9-Fluorenylmethoxycarbonyl (Fmoc) amino acids were first used for solid phase peptide synthesis a little more than a decade ago. Since that time, Fmoc solid phase peptide synthesis methodology has been greatly enhanced by the introduction of a variety of solid supports, linkages, and side chain protecting groups, as well as by increased understanding of solvation conditions. These advances have led to many impressive syntheses, such as those of biologically active and isotopically labeled peptides and small proteins. The great variety of conditions under which Fmoc solid phase peptide synthesis may be carried out represents a truly "orthogonal" scheme, and thus offers many unique opportunities for bioorganic chemistry.read more
Citations
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Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support
TL;DR: In this article, a series of predefined, short peptide sequences are assembled by manual or automated spotting of small aliquots of solutions containing the activated amino acid derivatives onto marked positions on the sheets.
Journal ArticleDOI
Biochemical Characterization of Human Collagenase-3
TL;DR: Analysis of the substrate specificity of collagenase-3 revealed that soluble type II collagen was preferentially hydrolyzed, while the enzyme was 5 or 6 times less efficient at cleaving type I or III collagen.
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A cleavage method which minimizes side reactions following Fmoc solid phase peptide synthesis.
TL;DR: Synthesis and cleavage of 10 peptides demonstrated the complementarity of Fmoc chemistry with Reagent K for efficient synthesis of complex peptides and assessed the relative effectiveness of various scavengers in suppressing side reactions.
Journal ArticleDOI
Development of fibrin derivatives for controlled release of heparin-binding growth factors
TL;DR: The results suggest that these matrices could serve as therapeutic materials to enhance peripheral nerve regeneration through nerve guide tubes and may have more general usefulness in tissue engineering.
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The cathelicidin anti-microbial peptide LL-37 is involved in re-epithelialization of human skin wounds and is lacking in chronic ulcer epithelium.
Johan Heilborn,Margareta Frohm Nilsson,Gunnar Kratz,Günther Weber,Ole E. Sørensen,Niels Borregaard,Mona Ståhle-Bäckdahl +6 more
TL;DR: It is suggested that, in addition to being an anti-microbial peptide, LL-37 also plays a part in wound closure and that its reduction in chronic wounds impairs re-epithelialization and may contribute to their failure to heal.
References
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Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides
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A Protein Sequenator
TL;DR: The protein sequenator is an instrument for the automatic determination of amino acid sequences in proteins and peptides that operates on the principle of the phenylisothiocyanate degradation scheme and has been applied to the whole molecule of apomyoglobin from the humpback whale.
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General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids
TL;DR: Through examination of the binding of these analogs to monoclonal antibodies raised against residues 75-110 of HA1, it was found that a single amino acid, aspartic acid at position 101, is of unique importance to the interaction.
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Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.
TL;DR: It was found that the leucine residues at positions 148 and 151 were essential for reaction with antisera raised against intact virus, and may lead to better understanding of the basis of antigen-antibody interaction and antibody specificity.