Journal ArticleDOI
Solid Phase Peptide Synthesis
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TLDR
This work has shown that effective methods of peptide synthesis are crucial to progress in this area, because only by synthesis can adequate amounts of important peptides be made available for chemical, biologi...Abstract:
The last two decades have been an era of rapid progress in peptide research. This era was begun by the work of Sanger on the amino acid sequence determination of insulin and by du Vigneaud on the structure determination and synthesis of oxytocin. This period has seen impressive progress in the structure elucidation and synthesis of many peptides of natural origin and of great biological significance, as well as in methods for sequence determination and chemical synthesis of peptides [1–4]. Perfection of techniques and instruments for automatic determination of the amino acid sequence of peptides and proteins has made possible a greatly broadened understanding of genetics and evolution as well as the more chemical areas of mechanism of action of enzymes and hormones, and physical chemistry of peptides and proteins. Effective methods of peptide synthesis are crucial to progress in this area, because only by synthesis can adequate amounts of important peptides be made available for chemical, biologi...read more
Citations
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Journal ArticleDOI
The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: Peptoid molecular transporters
Paul A. Wender,Dennis J. Mitchell,Kanaka Pattabiraman,Erin T. Pelkey,Lawrence Steinman,Jonathan B. Rothbard +5 more
TL;DR: Overall, a transporter has been developed that is superior to Tat(49-57), protease resistant, and more readily and economically prepared and suggest that the guanidinium groups of Tat( 49-57) play a greater role in facilitating cellular uptake than either charge or backbone structure.
Journal ArticleDOI
Rethinking amide bond synthesis
TL;DR: A new generation of amide-forming reactions are reviewed and summarize their potential application to current synthetic challenges, including the development of catalytic amide formation, the synthesis of therapeutic peptides and the preparation of modified peptide and proteins.
PatentDOI
Peptide nucleic acids
TL;DR: A peptide nucleic acid (PNA) as discussed by the authors is a class of compounds that can bind complementary ssDNA and RNA strands more strongly than a corresponding DNA, and it can be used to attach DNA bases to a peptide backbone through a suitable linker.
Patent
Secreted and Transmembrane Polypeptides and Nucleic Acids Encoding the Same
Kevin P. Baker,Maureen Beresini,Laura DeForge,Luc Desnoyers,Ellen Filvaroff,Wei-Qiang Gao,Mary E. Gerritsen,Audrey Goddard,Paul J. Godowski,Austin L. Gurney,Steven Sherwood,Victoria Smith,Timothy A. Stewart,Daniel Tumas,Colin K. Watanabe,William I. Wood,Zemin Zhang +16 more
TL;DR: In this paper, the present invention is directed to secreted and transmembrane polypeptides and to nucleic acid molecules encoding those polyptides, and vectors and host cells comprising those nucleic amino acid sequences.
Journal ArticleDOI
A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: Ramifications for the complex physiology of TNF
TL;DR: A novel, rapidly inducible cell surface cytotoxic integral transmembrane form of TNF is identified and characterized and it is suggested that cell borne cytokines and cytotoxins may be the primary mediators of directed inflammatory responses.
References
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Journal ArticleDOI
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Sidney Udenfriend,Stanley Stein,Peter Böhlen,Wallace Dairman,Willy Leimgruber,Manfred Weigele +5 more
TL;DR: Fluorescamine is a new reagent for the detection of primary amines in the picomole range that is almost instantaneous at room temperature in aqueous media and the products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent.
Journal ArticleDOI
Solid-phase peptide synthesis. 3. an improved synthesis of bradykinin.
Journal ArticleDOI
The Synthesis of Ribonuclease A
Bernd Gutte,R. B. Merrifield +1 more
TL;DR: A protected linear polypeptide of 124 amino acid residues with the sequence of bovine pancreatic ribonuclease A was synthesized by the solid phase method, showing the high substrate specificity to be expected of RNase A and indicating that the five NH2-terminal residues of S-protein are not required for the protein to oxidize and fold in the presence of s-peptides to give an active enzyme.