Journal ArticleDOI
Spectrophotometric determination of oxidized and reduced pyridine nucleotides in erythrocytes using a single extraction procedure.
TLDR
This work has developed a method for determining oxidized and reduced NAD and NADP in human erythrocytes using a single extract that eliminates the need for reducing agents and thus allows the use of a spectrophotometric cycling assay.About:
This article is published in Analytical Biochemistry.The article was published on 1987-08-01. It has received 176 citations till now. The article focuses on the topics: NAD+ kinase.read more
Citations
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Journal ArticleDOI
Sirt3 Mediates Reduction of Oxidative Damage and Prevention of Age-Related Hearing Loss under Caloric Restriction
Shinichi Someya,Shinichi Someya,Wei Yu,William C. Hallows,Jinze Xu,James M. Vann,Christiaan Leeuwenburgh,Masaru Tanokura,John M. Denu,Tomas A. Prolla +9 more
TL;DR: These findings suggest that Sirt3-dependent mitochondrial adaptations may be a central mechanism of aging retardation in mammals and suggest that CR reduces oxidative DNA damage in multiple tissues and prevents AHL in wild-type mice but fails to modify these phenotypes in mice lacking the mitochondrial deacetylase Sirt 3.
Journal ArticleDOI
AMPK regulates NADPH homeostasis to promote tumour cell survival during energy stress
TL;DR: It is shown that AMPK activation, during energy stress, prolongs cell survival by redox regulation and has a key function in NADPH maintenance, which is critical for cancer cell survival under energy stress conditions, such as glucose limitations, anchorage-independent growth and solid tumour formation in vivo.
Journal ArticleDOI
Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase.
Seung-Hee Jo,Mi-Kyung Son,Ho-Jin Koh,Su Min Lee,In-Hwan Song,Yong-Ou Kim,Young Sup Lee,Kyu-Shik Jeong,Won Bae Kim,Jeen-Woo Park,Byoung Joon Song,Tae-Lin Huh +11 more
TL;DR: It is demonstrated that IDPm is induced by ROS and that decreased expression of IDpm markedly elevates the ROS generation, DNA fragmentation, lipid peroxidation, and concurrent mitochondrial damage with a significant reduction in ATP level, and thus plays a key role in cellular defense against oxidative stress-induced damage.
Journal ArticleDOI
Cytosolic NADP(+)-dependent isocitrate dehydrogenase status modulates oxidative damage to cells.
Su Min Lee,Ho-Jin Koh,Ho-Jin Koh,Dong-Chan Park,Dong-Chan Park,Byoung Joon Song,Tae-Lin Huh,Tae-Lin Huh,Jeen-Woo Park +8 more
TL;DR: Direct evidence is provided correlating the activities of IDPc and the maintenance of the cellular redox state, suggesting that IDP c plays an important role in cellular defense against oxidative stress.
Journal ArticleDOI
SIRT3 Protein Deacetylates Isocitrate Dehydrogenase 2 (IDH2) and Regulates Mitochondrial Redox Status
TL;DR: This work provides direct biochemical and biological evidence establishing an exquisite regulatory relationship between IDH2 and SIRT3 under acute and chronic caloric restriction, and implicates Sirt3 as a potential regulator of IDH 2-dependent functions in cancer cell metabolism.
References
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Journal ArticleDOI
The measurement of pyridine nucleotides by enzymatic cycling.
TL;DR: This chapter describes the measurement of pyridine nucleotides by enzymatic cycling and it is found that as pyruvate formation during cycling is not strictly linear with DPN concentration, standards of DPN + are included which increase in steps of two to cover the range of assay.
Journal ArticleDOI
An improved cycling assay for nicotinamide adenine dinucleotide.
Carl Bernofsky,Marcella Swan +1 more
TL;DR: A new cycling assay for NAD that uses thiazolyl blue as a terminal electron acceptor has been found to offer significant advantages over the more established procedure that employs 2,6-dichlorophenolindophenol.
Journal ArticleDOI
Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells.
Vilberto Stocchi,Luigi Cucchiarini,Mauro Magnani,Laura Chiarantini,Pierangela Palma,G. Crescentini +5 more
TL;DR: A simple and rapid method for the determination of ATP, ADP, AMP, NADP+, NAD+, NADPH, and NADH in human erythrocytes is described and levels of adenine and pyridine nucleotides in normal adults are presented.
Journal ArticleDOI
The stability of pyridine nucleotides.
TL;DR: This paper is intended as a practical statement of the range of conditions of temperature and pH value tolerated by the acid and basic forms and can serve as a guide either for the destruction of the unwanted forms or for the preservation and storage of the desired forms.
Journal ArticleDOI
A simple ultramicro method for determination of pyridine nucleotides in tissues
J.S. Nisselbaum,Saul Green +1 more
TL;DR: A method which is relatively simple to perform, yet is precise and sensitive, and is capable for determining reduced or oxidized NAD+ or NADP+ in amounts as low as 1 × 10−12 mole per cuvet is submitted.