Journal ArticleDOI
Stabilization of dimeric β-glucosidase from Aspergillus niger via glutaraldehyde immobilization under different conditions.
Perla Guadalupe Vazquez-Ortega,Perla Guadalupe Vazquez-Ortega,Maria Teresa Alcaraz-Fructuoso,Juan Antonio Rojas-Contreras,Javier López-Miranda,Roberto Fernandez-Lafuente +5 more
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TLDR
The new biocatalysts are significantly more stable and avoid enzyme subunit desorption, being the immobilization pH a key point in their design.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2018-03-01. It has received 75 citations till now. The article focuses on the topics: Immobilized enzyme & Enzyme assay.read more
Citations
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Journal ArticleDOI
Parameters necessary to define an immobilized enzyme preparation
TL;DR: In this review, the reasons for loss of activity during immobilization are outlined and suitable diagnostic tests are highlighted to elucidate the precise cause and thereby methods to restore activity.
Journal ArticleDOI
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies
Rafael C. Rodrigues,Ángel Berenguer-Murcia,Diego Carballares,Roberto Morellon-Sterling,Roberto Fernandez-Lafuente +4 more
TL;DR: This review is focused on how enzyme immobilization may improve enzyme stability, and details other possibilities to stabilize enzymes: generation of favorable enzyme environments, prevention of enzyme subunit dissociation in multimeric enzymes, generation of more stable enzyme conformations, or enzyme rigidification via multipoint covalent attachment.
Journal ArticleDOI
On the taught new tricks of enzymes immobilization: An all-inclusive overview
TL;DR: This review describes the choices of support materials and cross-linkers together with several mechanisms that influence the performance, stabilization and hyperactivation of immobilized enzymes.
Journal ArticleDOI
Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization.
TL;DR: The pitfalls of using incorrectly designed immobilization protocols are highlighted and why in many cases sub-optimal results are obtained and solutions to overcome these challenges are described.
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Biotransformation of lignocellulosic biomass into industrially relevant products with the aid of fungi-derived lignocellulolytic enzymes.
Sara Saldarriaga-Hernandez,Carolina Velasco-Ayala,Paulina Leal-Isla Flores,Magdalena Rostro-Alanis,Roberto Parra-Saldívar,Hafiz M.N. Iqbal,Danay Carrillo-Nieves +6 more
TL;DR: The present review will discuss the classification and enzymatic degradation pathways of lignocellulolytic biomass as well as the potential and current industrial applications of the involved fungal enzymes.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
TL;DR: In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.
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Enzyme immobilisation in biocatalysis : Why, what and how
Roger A. Sheldon,Sander van Pelt +1 more
TL;DR: An overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented and emphasis is placed on relatively recent developments, such as the use of novel supports such as mesoporous silicas, hydrogels, and smart polymers, and cross-linked enzyme aggregates (CLEAs).
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Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
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Modifying enzyme activity and selectivity by immobilization.
Rafael C. Rodrigues,Claudia Ortiz,Ángel Berenguer-Murcia,Rodrigo Torres,Roberto Fernandez-Lafuente +4 more
TL;DR: In this tutorial review, some of the main reasons that may produce an improvement in enzyme activity, specificity or selectivity, either real or apparent, due to immobilization are listed.