Journal ArticleDOI
Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli
Basanta K. Ghora,David Apirion +1 more
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TLDR
E. coli contains at least three different sequence variants of 5S rRNA, all of which can be identified in the 9S transcript, indicating that 9S RNA is transcribed from most, if not all, of the active rRNA genes, and that RNAase E processes transcripts derived from all these r RNA genes.About:
This article is published in Cell.The article was published on 1978-11-01. It has received 241 citations till now. The article focuses on the topics: RNA & RNA editing.read more
Citations
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Journal ArticleDOI
A DEAD-box RNA helicase in the Escherichia coli RNA degradosome
TL;DR: It is suggested that RhlB acts by unwinding RNA structures that impede the processive activity of PNPase, and is thus an important enzyme in mRNA turnover.
Journal ArticleDOI
Copurification of E. coli RNAase E and PNPase: Evidence for a specific association between two enzymes important in RNA processing and degradation
Agamemnon J. Carpousis,Agamemnon J. Carpousis,Griet Van Houwe,Claude Pierre Ehretsmann,Henry M. Krisch +4 more
TL;DR: The discovery of a specific association between RNAase E and PNPase raises the intriguing possibility that these enzymes act cooperatively in the processing and degradation of RNA.
Journal ArticleDOI
Assembly of Bacterial Ribosomes
TL;DR: The history of the early work on ribosome assembly in Escherichia coli is presented, including a description of in vivo and in vitro intermediates, and the effects of ribosomal proteins in driving these events are explored.
Journal ArticleDOI
The Critical Role of RNA Processing and Degradation in the Control of Gene Expression
Cecília M. Arraiano,José M. Andrade,Susana Domingues,Inês Batista Guinote,Michal Malecki,Rute G. Matos,Ricardo N. Moreira,Vânia Pobre,Filipa P. Reis,Margarida Saramago,Inês J. Silva,Sandra C. Viegas +11 more
TL;DR: In bacteria, RNases act as a global regulatory network extremely important for the regulation of RNA levels, and intervene in the decay of many different mRNAs and small noncoding RNAs.
Journal ArticleDOI
RNase E: at the interface of bacterial RNA processing and decay
TL;DR: A new model to rationalize the mechanism of RNase E action in the context of its localization in the bacterial cell is proposed, which challenges traditional models in which soluble RNases are proposed to randomly interact with RNAs in the cytosol.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A two-dimensional fractionation procedure for radioactive nucleotides
TL;DR: High-voltage ionophoresis is used in both dimensions for the two-dimensional fractionation of ribonuclease digests of 32P-labelled RNA and the determination of the sequence of a nucleotide by partial digestion with spleen phosphodiesterase.
Journal ArticleDOI
The sequence of 5 s ribosomal ribonucleic acid.
TL;DR: The nucleotide sequence of the low molecular weight ribosomal RNA (5 s RNA) of Escherichia coli has been studied using 32P-labelled RNA and paper fractionation techniques and a new two-dimensional procedure suitable for fractionating oligonucleotides up to 25 residues long is described.
Book ChapterDOI
[10] Protein fractionation on the basis of solubility in aqueous solutions of salts and organic solvents
Journal ArticleDOI
Small Ribonucleic Acids of Escherichia coli I. CHARACTERIZATION BY POLYACRYLAMIDE GEL ELECTROPHORESIS AND FINGERPRINT ANALYSIS
TL;DR: Two-dimensional polyacrylamide gel electrophoresis was used to purify RNA in the 4 S to 5 S region and shows that during 20 min labeling the newly characterized molecules accumulate at levels comparable to many individual tRNAs.
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