Structure of histone-based chromatin in Archaea
Francesca Mattiroli,S. Bhattacharyya,Pamela N. Dyer,Alison E. White,Kathleen Sandman,Brett W. Burkhart,Kyle R. Byrne,Thomas Lee,Natalie G. Ahn,Thomas J. Santangelo,John N. Reeve,Karolin Luger,Karolin Luger,Karolin Luger +13 more
TLDR
It is established that the histone-based mechanism of DNA compaction predates the nucleosome, illuminating the origin of the nucleOSome.Abstract:
Small basic proteins present in most Archaea share a common ancestor with the eukaryotic core histones. We report the crystal structure of an archaeal histone-DNA complex. DNA wraps around an extended polymer, formed by archaeal histone homodimers, in a quasi-continuous superhelix with the same geometry as DNA in the eukaryotic nucleosome. Substitutions of a conserved glycine at the interface of adjacent protein layers destabilize archaeal chromatin, reduce growth rate, and impair transcription regulation, confirming the biological importance of the polymeric structure. Our data establish that the histone-based mechanism of DNA compaction predates the nucleosome, illuminating the origin of the nucleosome.read more
Citations
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Chromosome organization in bacteria: mechanistic insights into genome structure and function
TL;DR: The hierarchical nature of bacterial chromosome structure and how it is influenced by diverse types of nucleoid-associated proteins is discussed, including in gene expression, chromosome segregation and cell cycle regulation.
Journal ArticleDOI
Primary Role of the Nucleosome
Roger D. Kornberg,Yahli Lorch +1 more
TL;DR: The nucleosome serves not only as a general gene repressor, but also as a repressor of all transcription (genic, intragenic, and intergenic) in the regulation of transcription.
Journal ArticleDOI
Old cogs, new tricks: the evolution of gene expression in a chromatin context.
TL;DR: The extent to which transcriptional regulatory components function in the context of the evolutionarily ancient role of chromatin as a barrier to processes acting on DNA is explored and how chromatin proteins have diversified to carry out evolutionarily recent functions that accompanied the emergence of differentiation and development in multicellular eukaryotes is discussed.
Journal ArticleDOI
Histone variants at a glance.
Paul B. Talbert,Steven Henikoff +1 more
TL;DR: In this paper, the authors briefly examine new insights into histone origins and discuss variants from each of the histone families, focusing on how structural differences may alter their functions, including DNA repair, chromosome segregation and regulation of transcription initiation.
Journal ArticleDOI
Mosaic origin of the eukaryotic kinetochore
Eelco C. Tromer,Eelco C. Tromer,Eelco C. Tromer,Jolien J. E. van Hooff,Jolien J. E. van Hooff,Geert J. P. L. Kops,Geert J. P. L. Kops,Berend Snel +7 more
TL;DR: The origins of the kinetochore before the last eukaryotic common ancestor (LECA) are investigated using phylogenetic trees, sensitive profile-versus-profile homology detection, and structural comparisons of its protein components.
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