Journal ArticleDOI
Studies on the purification and properties of factor Tu from E. coli.
Reads0
Chats0
TLDR
Transfer factor Tu has been purified to apparent homogeneity from extracts of Escherichia coli and contains a sulfhydryl group essential for GDP binding, which in Tu free of nucleotide reacts readily with N-ethylmaleimide, but in Tu-GDP is almost inert.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-11-01. It has received 198 citations till now. The article focuses on the topics: Dissociation constant.read more
Citations
More filters
Journal ArticleDOI
Discrimination between aminoacyl groups on su+7 tRNA by elongation factor Tu
Robert G. Knowlton,Michael Yarus +1 more
TL;DR: Results show that EF Tu can distinguish between two aminoacyl-tRNAs which differ only in the aminoacyls group, and implications for the unusual amino acid specificity of su + 7 tRNA are discussed.
Journal ArticleDOI
Studies on the ribosomal sites involved in factors Tu and G-dependent reactions.
TL;DR: These studies have examined the effect of the antibiotic on aminoacyl-tRNA binding from the ternary complex to the 30S particle, and the results show that thiostrepton inhibits binding by interacting with the 50S ribosomal subunit.
Journal ArticleDOI
Specific interaction of anticodon loop residues with yeast phenylalanyl-tRNA synthetase.
TL;DR: Data suggest a sequence-specific contact between the anticodon of yeast tRNAPhe and the cognate synthetase and the level of aminoacylation was very different for different sequences inserted.
Journal ArticleDOI
Kinetics and thermodynamics of the interaction of elongation factor Tu with elongation factor Ts, guanine nucleotides, and aminoacyl-tRNA.
TL;DR: A complete mechanism to explain the interactions among EF-Tu, EF-Ts, guanine nucleotides, and aminoacyl-tRNA has been developed.
Journal ArticleDOI
On the biological role of ribosomal protein BM-L11 of Bacillus megaterium, homologous with Escherichia coli ribosomal protein L11
TL;DR: Ribosomes from a thiostrepton-resistant mutant of Bacillus megaterium lack a protein, BM-L11, which is homologous with Escherichia coli ribosomal protein L11, but such ribosomes retain partial activity in cell-free synthesis of polyphenylalanine and can be restored to full activity by reconstitution with protein BM- L11.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
Spectroscopic determination of tryptophan and tyrosine in proteins.
TL;DR: A procedure is presented which strongly reduces or elimi- nates these interactions, normalizes their absorption, and consequently permits a more precise analysis of tryptophan and tyrosine in proteins.