Journal ArticleDOI
Study of grain cell wall structures by microscopic analysis with four different staining techniques
Emmie Dornez,Ulla Holopainen,Sven Cuyvers,Kaisa Poutanen,Jan A. Delcour,Christophe M. Courtin,Emilia Nordlund +6 more
TLDR
Four different grain cell wall staining techniques were compared and AX was mostly concentrated in nucellar epidermis and aleurone cells, whereas β-glucan was concentrated more in subaleur one cells, giving new insight into the structure of the cereal dietary fiber complex.About:
This article is published in Journal of Cereal Science.The article was published on 2011-08-12. It has received 62 citations till now. The article focuses on the topics: Aleurone & Endosperm.read more
Citations
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Journal ArticleDOI
Endosperm and aleurone cell structure in barley and wheat as studied by optical and Raman microscopy
TL;DR: In this paper, the authors used optical and Raman microscopy to localize wheat and barley grain components using selective stains and autofluorescence to image the ferulic acid and other fluorescing substances.
Journal ArticleDOI
Element distribution and iron speciation in mature wheat grains (Triticum aestivum L.) using synchrotron X-ray fluorescence microscopy mapping and X-ray absorption near-edge structure (XANES) imaging.
Niels De Brier,Sara Gomand,Erica Donner,David L. Paterson,Erik Smolders,Jan A. Delcour,Enzo Lombi +6 more
TL;DR: Speciation analysis suggests that Fe is bound to nicotianamine rather than phytate in the nucellar projection, and that more complex Fe structures may also be present, as well as revealing the complexity of the physiological processes responsible for element accumulation and bioaccessibility.
Journal ArticleDOI
Impact of hydrothermal pre-treatment to chemical composition, enzymatic digestibility and spatial distribution of cell wall polymers.
Ulla Holopainen-Mantila,Kaisa Marjamaa,Zara Merali,Andres Käsper,Peter de Bot,Anna Stiina Jääskeläinen,Keith W. Waldron,Kristiina Kruus,Tarja Tamminen +8 more
TL;DR: The sample with the highest digestibility was observed to have intensified cellulose staining, possibly reflecting the increased accessibility of cellulose, and the highest yields were obtained from the samples with a low xylan and diferulate content.
Journal ArticleDOI
Quantitative structural organisation model for wheat endosperm cell walls: Cellulose as an important constituent.
Ghanendra Gartaula,Sushil Dhital,Gabriele Netzel,Bernadine M. Flanagan,Gleb E. Yakubov,Cherie T. Beahan,Helen Collins,Rachel A. Burton,Antony Bacic,Michael J. Gidley +9 more
TL;DR: A model for endosperm cell wall structural organisation is proposed, based on a core of cellulose and interacting non-cellulosic polysaccharides which anchors AX that in turn retains MLGs through physical entanglement.
Journal ArticleDOI
Iron and zinc complexation in wild-type and ferritin-expressing wheat grain: implications for mineral transport into developing grain
Andrew L. Neal,Kalotina Geraki,Søren Borg,Paul D. Quinn,J. Fred W. Mosselmans,Henrik Brinch-Pedersen,Peter R. Shewry +6 more
TL;DR: Derivative X-ray absorption spectra suggest that mineral complexation in the transfer region of ferritin-expressing grains is quite different from that in wild-type grain, which may explain why the raised levels of minerals transported to the developing grain accumulate within the crease region of the transgenic grain.
References
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Journal ArticleDOI
Crystallographic analysis shows substrate binding at the -3 to +1 active site subsites and at the surface of glycoside hydrolase family 11 endo-1,4-{beta}-xylanases
Elien Vandermarliere,Tine M. Bourgois,Sigrid Rombouts,Steven Van Campenhout,Guido Volckaert,Sergei V. Strelkov,Jan A. Delcour,Anja Rabijns,Christophe M. Courtin +8 more
TL;DR: The existence of an unproductive second ligand-binding site located on the surface of both the B. subtilis and A. niger xylanases was observed, suggesting that this extra binding site may have a function similar to the separate carbohydrate-binding modules of other glycoside hydrolase families.
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Wheat endosperm cell walls: Spatial heterogeneity of polysaccharide structure and composition using micro-scale enzymatic fingerprinting and FT-IR microspectroscopy
Luc Saulnier,Paul Robert,Mathilde Grintchenko,Frédéric Jamme,Brigitte Bouchet,Fabienne Guillon +5 more
TL;DR: In this paper, micro-scale enzymatic fingerprinting and FT-IR microspectroscopy were used to investigate changes of polysaccharide structure and composition in cell walls from wheat endosperm.
Journal ArticleDOI
Deposition of cell wall polysaccharides in wheat endosperm during grain development: Fourier transform-infrared microspectroscopy study.
TL;DR: Dramatic changes in endosperm cell wall composition were detected during wheat grain development with respect to both the relative occurrence of individual constituents and the fine structure of the AX.
Journal ArticleDOI
The effect of environment on endosperm cell-wall development in Triticum aestivum during grain filling: an infrared spectroscopic imaging study
Geraldine A. Toole,Reginald H. Wilson,Mary L. Parker,Nikolaus Wellner,Tim Wheeler,Peter R. Shewry,E. N. C. Mills +6 more
TL;DR: Two bread-making wheat cultivars, Spark and Rialto, were selected and studies show that the structure of the arabinoxylans in the endosperm cell walls changes from a highly branched form to a less brancher form, with differences in the rate of restructuring occurring between the two cultivars.