Journal ArticleDOI
Study of grain cell wall structures by microscopic analysis with four different staining techniques
Emmie Dornez,Ulla Holopainen,Sven Cuyvers,Kaisa Poutanen,Jan A. Delcour,Christophe M. Courtin,Emilia Nordlund +6 more
TLDR
Four different grain cell wall staining techniques were compared and AX was mostly concentrated in nucellar epidermis and aleurone cells, whereas β-glucan was concentrated more in subaleur one cells, giving new insight into the structure of the cereal dietary fiber complex.About:
This article is published in Journal of Cereal Science.The article was published on 2011-08-12. It has received 62 citations till now. The article focuses on the topics: Aleurone & Endosperm.read more
Citations
More filters
Journal ArticleDOI
Endosperm and aleurone cell structure in barley and wheat as studied by optical and Raman microscopy
TL;DR: In this paper, the authors used optical and Raman microscopy to localize wheat and barley grain components using selective stains and autofluorescence to image the ferulic acid and other fluorescing substances.
Journal ArticleDOI
Element distribution and iron speciation in mature wheat grains (Triticum aestivum L.) using synchrotron X-ray fluorescence microscopy mapping and X-ray absorption near-edge structure (XANES) imaging.
Niels De Brier,Sara Gomand,Erica Donner,David L. Paterson,Erik Smolders,Jan A. Delcour,Enzo Lombi +6 more
TL;DR: Speciation analysis suggests that Fe is bound to nicotianamine rather than phytate in the nucellar projection, and that more complex Fe structures may also be present, as well as revealing the complexity of the physiological processes responsible for element accumulation and bioaccessibility.
Journal ArticleDOI
Impact of hydrothermal pre-treatment to chemical composition, enzymatic digestibility and spatial distribution of cell wall polymers.
Ulla Holopainen-Mantila,Kaisa Marjamaa,Zara Merali,Andres Käsper,Peter de Bot,Anna Stiina Jääskeläinen,Keith W. Waldron,Kristiina Kruus,Tarja Tamminen +8 more
TL;DR: The sample with the highest digestibility was observed to have intensified cellulose staining, possibly reflecting the increased accessibility of cellulose, and the highest yields were obtained from the samples with a low xylan and diferulate content.
Journal ArticleDOI
Quantitative structural organisation model for wheat endosperm cell walls: Cellulose as an important constituent.
Ghanendra Gartaula,Sushil Dhital,Gabriele Netzel,Bernadine M. Flanagan,Gleb E. Yakubov,Cherie T. Beahan,Helen Collins,Rachel A. Burton,Antony Bacic,Michael J. Gidley +9 more
TL;DR: A model for endosperm cell wall structural organisation is proposed, based on a core of cellulose and interacting non-cellulosic polysaccharides which anchors AX that in turn retains MLGs through physical entanglement.
Journal ArticleDOI
Iron and zinc complexation in wild-type and ferritin-expressing wheat grain: implications for mineral transport into developing grain
Andrew L. Neal,Kalotina Geraki,Søren Borg,Paul D. Quinn,J. Fred W. Mosselmans,Henrik Brinch-Pedersen,Peter R. Shewry +6 more
TL;DR: Derivative X-ray absorption spectra suggest that mineral complexation in the transfer region of ferritin-expressing grains is quite different from that in wild-type grain, which may explain why the raised levels of minerals transported to the developing grain accumulate within the crease region of the transgenic grain.
References
More filters
Journal ArticleDOI
Probing the cell wall heterogeneity of micro-dissected wheat caryopsis using both active and inactive forms of a GH11 xylanase.
Johnny Beaugrand,Gabriel Paës,Danièle Reis,Masayuki Takahashi,Philippe Debeire,Michael J. O’Donohue,Brigitte Chabbert +6 more
TL;DR: The data obtained showed that the micro-dissected layers provided an increased accessible surface for the xylanase and that the enzyme-targeted cell walls were penetrated more quickly than those in intact bran and the cell wall network by itself physically restricts enzyme penetration.
Journal ArticleDOI
Inactive fluorescently labeled xylanase as a novel probe for microscopic analysis of arabinoxylan containing cereal cell walls
Emmie Dornez,Sven Cuyvers,Ulla Holopainen,Emilia Nordlund,Kaisa Poutanen,Jan A. Delcour,Christophe M. Courtin +6 more
TL;DR: A new technique to visualize cereal cell walls by fluorescence microscopy was developed based on an inactive fluorescently labeled xylanase binding to arabinoxylan, an important polysaccharide in grain cell walls in terms of the technological and physiological functionalities of grain.
Journal ArticleDOI
Specificity of monoclonal antibodies generated against arabinoxylans of cereal grains
José Juan Ordaz-Ortiz,Fabienne Guillon,Olivier Tranquet,Gaud Dervilly-Pinel,Vinh Tran,Luc Saulnier +5 more
TL;DR: ELISA competition tests and molecular modelling suggest that the conformation adopted by beta-(1→4) linked xylose residues is an antigenic determinant recognized by the different antibodies.
Journal ArticleDOI
Aleurone cell walls of wheat grain: high spatial resolution investigation using synchrotron infrared microspectroscopy.
TL;DR: The high spectral quality obtained in the small analyzed domain has been beneficial in estimating the relative proportions of β-glucan and arabinoxylan, through the use of principal component analysis (PCA).
Journal ArticleDOI
Variability in the Structure of Rye Flour Alkali-Extractable Arabinoxylans
TL;DR: Results indicated that the rye flour alkali-extractable arabinoxylan (AE-AX) consist of a continuum of structures rather than of two types of AX or two type of regions in the AX molecule.