Substrate recognition and catalysis by the exoribonuclease RNase R.
TLDR
A model for how RNase R interacts with its substrates and degrades RNA is presented and it is shown that ribose moieties are required for recognition of the substrate as a whole sinceRNase R is unable to bind or degrade single-stranded DNA.About:
This article is published in Journal of Biological Chemistry.The article was published on 2006-10-06 and is currently open access. It has received 221 citations till now. The article focuses on the topics: RNase R & RNase PH.read more
Citations
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Cell-type specific features of circular RNA expression.
TL;DR: Using an improved computational approach for circular RNA identification, widespread circular RNA expression is found in Drosophila melanogaster and it is estimated that in humans, circular RNA may account for 1% as many molecules as poly(A) RNA.
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Circular RNAs: diversity of form and function
Erika Lasda,Roy Parker +1 more
TL;DR: The breadth of circular RNAs, their biogenesis and metabolism, and their known and anticipated functions are reviewed.
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Circular RNAs: analysis, expression and potential functions.
Steven P. Barrett,Julia Salzman +1 more
TL;DR: This Primer outlines the discovery, roles and regulation of circular RNAs, focussing on their potential functions during development and on the regulation of and functional roles played by these molecules.
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Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/β-catenin pathway
Fang-Fang Li,Liyuan Zhang,Wei Li,Jieqiong Deng,Jian Zheng,Mingxing An,Jiachun Lu,Yifeng Zhou +7 more
TL;DR: Results indicate that cir-ITCH may have an inhibitory effect on ESCC by regulating the Wnt pathway.
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Circular RNA Is Expressed across the Eukaryotic Tree of Life
Peter L. Wang,Yun Bao,Muh-Ching Yee,Steven P. Barrett,Gregory J. Hogan,Mari Olsen,José R. Dinneny,Patrick O. Brown,Julia Salzman +8 more
TL;DR: It is reported that circular RNA isoforms are found in diverse species whose most recent common ancestor existed more than one billion years ago: fungi, plants, a plant, and protists, including S. pombe, which may be an ancient, conserved feature of eukaryotic gene expression programs.
References
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Exoribonuclease superfamilies: structural analysis and phylogenetic distribution
Yuhong Zuo,Murray P. Deutscher +1 more
TL;DR: This analysis analyzes the structure and phylogenetic distribution of the known exoribonucleases and identifies common motifs that can be used to characterize newly-discovered exorIBonucle enzymes, and correct some previously misassigned proteins.
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Degradation of RNA in bacteria: comparison of mRNA and stable RNA
TL;DR: In this review, each of these processes of decay of mRNA and degradation of stable RNA share many common features, and that their initial steps also overlap with those of RNA maturation.
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Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12
TL;DR: The demonstration that RNase II and polynucleotide phosphorylase (PNPase) are required for cell viability and mRNA turnover in Escherichia coli is demonstrated.
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A double-filter method for nitrocellulose-filter binding: application to protein-nucleic acid interactions
Isaac Wong,Timothy M. Lohman +1 more
TL;DR: The use of a 96-well dot-blot apparatus and the addition of a DEAE membrane beneath the nitrocellulose membrane improve precision and accuracy and permit a more accurate quantitation of filter-retention efficiency and nonspecific background retention based on free DNA rather than total DNA filtered.
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An Important Role for RNase R in mRNA Decay
TL;DR: It is shown here that another enzyme, RNase R, also participates in the process of mRNA decay, and is particularly important for removing mRNA fragments with extensive secondary structure, such as those derived from the many mRNAs that contain REP elements.