Journal ArticleDOI
Surface-structured bacterial cellulose with guided assembly-based biolithography (GAB).
Simone Bottan,Francesco Robotti,Prageeth Jayathissa,Alicia Hegglin,Nicolas Bahamonde,José A. Heredia-Guerrero,Ilker S. Bayer,Alice Scarpellini,Hannes Merker,Nicole Lindenblatt,Dimos Poulikakos,Aldo Ferrari +11 more
TLDR
The deployment of surface-structured bacterial cellulose substrates in model animals as skin wound dressing or body implant further proves the high durability and low inflammatory response to the material over a period of 21 days, demonstrating beneficial effects of surface structure on skin regeneration.Abstract:
A powerful replica molding methodology to transfer on-demand functional topographies to the surface of bacterial cellulose nanofiber textures is presented. With this method, termed guided assembly-based biolithography (GAB), a surface-structured polydimethylsiloxane (PDMS) mold is introduced at the gas-liquid interface of an Acetobacter xylinum culture. Upon bacterial fermentation, the generated bacterial cellulose nanofibers are assembled in a three-dimensional network reproducing the geometric shape imposed by the mold. Additionally, GAB yields directional alignment of individual nanofibers and memory of the transferred geometrical features upon dehydration and rehydration of the substrates. Scanning electron and atomic force microscopy are used to establish the good fidelity of this facile and affordable method. Interaction of surface-structured bacterial cellulose substrates with human fibroblasts and keratinocytes illustrates the efficient control of cellular activities which are fundamental in skin wound healing and tissue regeneration. The deployment of surface-structured bacterial cellulose substrates in model animals as skin wound dressing or body implant further proves the high durability and low inflammatory response to the material over a period of 21 days, demonstrating beneficial effects of surface structure on skin regeneration.read more
Citations
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Nanocellulose as a natural source for groundbreaking applications in materials science: Today’s state
Dieter Klemm,Emily D. Cranston,Dagmar Fischer,Miguel Gama,Stephanie A. Kedzior,Dana Kralisch,Friederike Kramer,Tetsuo Kondo,Tom Lindström,Sandor Nietzsche,Katrin Petzold-Welcke,Falk Rauchfuß +11 more
TL;DR: Nanocelluloses are natural materials with at least one dimension in the nano-scale as discussed by the authors, which combine important cellulose properties with the features of nanomaterials and open new horizons for materials science and its applications.
Journal ArticleDOI
Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions
Ute Römling,Michael Y. Galperin +1 more
TL;DR: The organization of four principal types of cellulose synthase operon found in various bacterial genomes are reviewed, additional bcs genes that encode components of the cellulose biosynthesis and secretion machinery are identified, and a unified nomenclature for these genes and subunits are proposed.
Journal ArticleDOI
Bacterial cellulose production, properties and applications with different culture methods: A review
TL;DR: The aims of this review are to provide an overview of the production of BC from different culture methods, to analyze the characteristics of particular BC productions, to indicate existing problems associated with different methods, and to choose suitable culture approaches for BC applications in different fields.
Journal ArticleDOI
Bacterial cellulose as a material for wound treatment: Properties and modifications. A review
TL;DR: This review highlights possible pathways for functionalization of BC, affecting all levels of its structural organization, including its biocompatibility, water uptake and release, and antimicrobial activity.
Journal ArticleDOI
3D printing of bacteria into functional complex materials
Manuel Schaffner,Patrick A. Rühs,Fergal Coulter,Fergal Coulter,Samuel Kilcher,André R. Studart +5 more
TL;DR: 3D printing of bacteria-laden hydrogels enables the digital fabrication of complex functional materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
References
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Journal ArticleDOI
Cellulose: Fascinating Biopolymer and Sustainable Raw Material
TL;DR: The current knowledge in the structure and chemistry of cellulose, and in the development of innovative cellulose esters and ethers for coatings, films, membranes, building materials, drilling techniques, pharmaceuticals, and foodstuffs are assembled.
Journal ArticleDOI
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
TL;DR: The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.
Journal ArticleDOI
Regulation of Wound Healing by Growth Factors and Cytokines
Sabine Werner,Richard Grose +1 more
TL;DR: This review summarizes the results of expression studies that have been performed in rodents, pigs, and humans to localize growth factors and their receptors in skin wounds and reports on genetic studies addressing the functions of endogenous growth factors in the wound repair process.
Journal ArticleDOI
Designing materials for biology and medicine
Robert Langer,David A. Tirrell +1 more
TL;DR: New challenges and directions in biomaterials research are discussed, including synthetic replacements for biological tissues, designing materials for specific medical applications, and materials for new applications such as diagnostics and array technologies.
Journal ArticleDOI
The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
Matthew J. Dalby,Nikolaj Gadegaard,Rahul S. Tare,Abhay Andar,Mathis O. Riehle,Pawel Herzyk,Chris D. W. Wilkinson,Richard O.C. Oreffo +7 more
TL;DR: The use of nanoscale disorder is demonstrated to stimulate human mesenchymal stem cells (MSCs) to produce bone mineral in vitro, in the absence of osteogenic supplements, which has implications for cell therapies.