Surface tension and viscosity of protein condensates quantified by micropipette aspiration
Huan Wang,Fleurie M. Kelley,Dragomir Milovanovic,Benjamin S. Schuster,Zheng Shi +4 more
- Vol. 1, Iss: 1, pp 100011
TLDR
A micropipette-based technique is developed that uniquely, to the knowledge, allows quantifications of both the surface tension and viscosity of biomolecular condensates, independent of labeling and surface-wetting effects.Abstract:
The material properties of biomolecular condensates have been suggested to play important biological and pathological roles. Despite the rapid increase in the number of biomolecules identified that undergo liquid-liquid phase separation, quantitative studies and direct measurements of the material properties of the resulting condensates have been severely lagging behind. Here, we develop a micropipette-based technique that uniquely, to our knowledge, allows quantifications of both the surface tension and viscosity of biomolecular condensates, independent of labeling and surface-wetting effects. We demonstrate the accuracy and versatility of this technique by measuring condensates of LAF-1 RGG domains and a polymer-based aqueous two-phase system. We further confirm our measurements using established condensate fusion and fluorescence recovery after photobleaching assays. We anticipate the micropipette-based technique will be widely applicable to biomolecular condensates and will resolve several limitations regarding current approaches.read more
Citations
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Capillary forces generated by biomolecular condensates
Bernardo Gouveia,Yoonji Kim,Joshua W. Shaevitz,Sabine Petry,Howard A. Stone,Clifford P. Brangwynne +5 more
TL;DR: The physical principles of capillarity are presented, including examples of how capillary forces structure multiphase condensates and remodel biological substrates.
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Endocytosis of Coacervates into Liposomes
Tiemei Lu,Susanne Liese,Ludo Schoenmakers,Christoph Weber,Hiroaki Suzuki,Wilhelm T. S. Huck,Evan Spruijt +6 more
TL;DR: Study of how the interaction between complex coacervates and liposomes as model systems can lead to wetting, membrane deformation, and endocytosis can help to better understand condensate–membrane interactions in cellular systems and provide new avenues for intracellular delivery using coacers.
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Phase Transitions of Associative Biomacromolecules.
TL;DR: In this article , the key concepts of phase transitions of aqueous solutions of associative biomacromolecules, specifically proteins that include folded domains and intrinsically disordered regions, are reviewed.
Journal ArticleDOI
Rheology and Viscoelasticity of Proteins and Nucleic Acids Condensates
TL;DR: The state-of-the-art of this quickly emerging field focusing on the material and rheological properties of protein condensates is reviewed and potential future directions and opportunities for interdisciplinary cross-talk between chemists, physicists, and biologists are highlighted.
Journal ArticleDOI
Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching
Fernando Muzzopappa,Johan Hummert,Michela Anfossi,Stanimir A. Tashev,Dirk-Peter Herten,Fabian Erdel +5 more
TL;DR: In this article , a workflow termed model-free calibrated half-FRAP (MOCHA-frAP) is introduced to probe the barrier at the condensate interface that is responsible for preferential internal mixing.
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