Journal ArticleDOI
Synthesis of nucleoside-3',5'-cyclic phosphorothioates by cyclothiophosphorylation of unprotected nucleosides
TLDR
In this paper, unprotected nucleosides were thiophosphorylated with thlophosphorousl chloride in trlalkyl phosphates and directly cycllzed by alkali hydroxide in aqueous acetonftrile to give the diaslereomers 3, 5'- cyclic cyclic phosphorothioates in good yields.About:
This article is published in Tetrahedron Letters.The article was published on 1988-01-01. It has received 38 citations till now. The article focuses on the topics: Alkali hydroxide & Nucleoside.read more
Citations
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Journal ArticleDOI
Cyclic nucleotide analogs as biochemical tools and prospective drugs.
TL;DR: This review will focus mainly on recent developments in cyclic nucleotide-related biochemical and pharmacological research, but also highlights some historical findings in the field.
Journal ArticleDOI
Novel (Rp)-cAMPS analogs as tools for inhibition of cAMP-kinase in cell culture. Basal cAMP-kinase activity modulates interleukin-1 beta action.
Bjørn Tore Gjertsen,Gunnar Mellgren,A.D. Otten,Erik Maronde,Hans G. Genieser,Bernd Jastorff,Olav Karsten Vintermyr,G S McKnight,Stein Ove Døskeland +8 more
TL;DR: It is concluded that the basal cAMP-kinase activity exerts a tonic inhibition of hepatocyte replication and can have a permissive role for the action of another (interleukin-1β) signaling pathway.
Journal ArticleDOI
Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine 3',5'-cyclic phosphorothioates.
TL;DR: Data show that the Rp and Sp substitutions are tolerated differently by the two intrachain sites of isozymes I and II, and support the hypothesis that it is the axial, and not the previously proposed equatorial oxygen that contributes the negative charge for the ionic interaction with an invariant arginine in all four binding sites.
Journal ArticleDOI
(Rp)-8-pCPT-cGMPS, a novel cGMP-dependent protein kinase inhibitor
TL;DR: The data obtained suggest that (Rp)-8-pCPT-cGMPS may be a useful tool for studying the role of cGMP in vitro and in intact cells.
Journal ArticleDOI
Inhibition of cGMP-dependent protein kinase by (Rp)-guanosine 3',5'-monophosphorothioates.
TL;DR: Rp‐8‐Cl‐cGMPS appears to be a rather selective inhibitor of the cGMP‐dependent protein kinase and may be a useful tool for studying the role of cG MP in broken and intact cell systems.
References
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Journal ArticleDOI
A novel method for phosphorylation of nucleosides to 5'-nucleotides
Journal ArticleDOI
Competitive cAMP antagonists for cAMP-receptor proteins.
P. J. M. Van Haastert,R. van Driel,Bernd Jastorff,Janina Baraniak,Wojciech J. Stec,R J De Wit +5 more
TL;DR: In this article, two diastereoisomers are known for each derivative: (SP)-cAMPPS, (RP)-cAMPS, and (SP-cAMPN(CH3)2, with apparent inhibition constants between 0.8 and 8 microM.
Journal ArticleDOI
The role of cAMP in nerve growth factor-promoted neurite outgrowth in PC12 cells.
C Richter-Landsberg,B Jastorff +1 more
TL;DR: The morphological differentiation of PC12 cells stimulated by NGF does not require cAMP as a second messenger and the constant increase of intracellular cAMP, caused by either forskolin or cAMP and the analogues, in combination with NGF, not only rapidly stimulated early neurite outgrowth but also exerted a maintaining effect on the neuronal network established by N GF.
Journal ArticleDOI
Adenosine 5'-phosphorothioate. A nucleotide analog that is a substrate, competitive inhibitor, or regulator of some enzymes that interact with adenosine 5'-phosphate
Andrew W. Murray,M. R. Atkinson +1 more
Journal ArticleDOI
Inhibitory action of certain cyclophosphate derivatives of cAMP on cAMP-dependent protein kinases
René J.W. de Wit,Doeke Hekstra,Bernd Jastorff,Wojciech J. Stec,Janina Baraniak,Roel van Driel,Peter J.M. van Haastert +6 more
TL;DR: The present work using highly purified compounds suggests the absence of a charge interaction, since the uncharged analog (Sp)-cAMPN(CH3)2 activates the kinase effectively.