Journal ArticleDOI
Synthetic gene network for entraining and amplifying cellular oscillations.
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TLDR
The ability to couple naturally occurring genetic oscillations to a synthetically designed network could lead to possible strategies for entraining and/or amplifying oscillations in cellular protein levels.Abstract:
We present a model for a synthetic gene oscillator and consider the coupling of the oscillator to a periodic process that is intrinsic to the cell. We investigate the synchronization properties of the coupled system, and show how the oscillator can be constructed to yield a significant amplification of cellular oscillations. We reduce the driven oscillator equations to a normal form, and analytically determine the amplification as a function of the strength of the cellular oscillations. The ability to couple naturally occurring genetic oscillations to a synthetically designed network could lead to possible strategies for entraining and/or amplifying oscillations in cellular protein levels.read more
Citations
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Journal ArticleDOI
A fast, robust and tunable synthetic gene oscillator
Jesse Stricker,Scott Cookson,Matthew R. Bennett,William H. Mather,Lev S. Tsimring,Jeff Hasty +5 more
TL;DR: An engineered genetic oscillator in Escherichia coli is described that is fast, robust and persistent, with tunable oscillatory periods as fast as 13 min, and Computational modelling demonstrates that the key design principle for constructing a robust oscillator is a time delay in the negative feedback loop.
Journal ArticleDOI
Principles of genetic circuit design
TL;DR: In this article, a review describes new tools that aid the construction of genetic circuits and discusses the failure modes encountered when assembling circuits, quantify their impact on performance, and review mitigation efforts.
Journal ArticleDOI
Engineered gene circuits
TL;DR: Synthetic gene networks will lead to new logical forms of cellular control, which could have important applications in functional genomics, nanotechnology, and gene and cell therapy.
Journal ArticleDOI
Oscillations and variability in the p53 system
Naama Geva-Zatorsky,Nitzan Rosenfeld,Shalev Itzkovitz,Ron Milo,Alex Sigal,Erez Dekel,Talia Yarnitzky,Yuvalal Liron,Paz Polak,Galit Lahav,Uri Alon +10 more
TL;DR: A view of the extensive variability of the behavior of a protein circuit in living human cells, both from cell to cell and in the same cell over time is provided.
References
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Journal ArticleDOI
On the Nature of Allosteric Transitions: A Plausible Model
TL;DR: "It is certain that all bodies whatsoever, though they have no sense, yet they have perception, and whether the body be alterant or alterec, evermore a perception precedeth operation; for else all bodies would be like one to another."
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A synthetic oscillatory network of transcriptional regulators
TL;DR: This work used three transcriptional repressor systems that are not part of any natural biological clock to build an oscillating network, termed the repressilator, in Escherichia coli, which periodically induces the synthesis of green fluorescent protein as a readout of its state in individual cells.
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Construction of a genetic toggle switch in Escherichia coli
TL;DR: The construction of a genetic toggle switch is presented—a synthetic, bistable gene-regulatory network—in Escherichia coli and a simple theory is provided that predicts the conditions necessary for bistability.
Journal ArticleDOI
Stochastic kinetic analysis of developmental pathway bifurcation in phage lambda -infected escherichia coli cells
TL;DR: The fraction of infected cells selecting the lysogenic pathway at different phage:cell ratios, predicted using a molecular-level stochastic kinetic model of the genetic regulatory circuit, is consistent with experimental observations.
Journal ArticleDOI
Engineering stability in gene networks by autoregulation
Attila Becskei,Luis Serrano +1 more
TL;DR: Simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli are designed and constructed and the gain of stability produced by negative feedback is shown.