Journal ArticleDOI
Tensile mechanical properties of three-dimensional type I collagen extracellular matrices with varied microstructure.
TLDR
Fundamental information regarding the 3-D microstructural-mechanical properties of the ECM and its component molecules are important to the overall understanding of cell-ECM interactions and the development of novel strategies for tissue repair and replacement.Abstract:
The importance and priority of specific micro-structural and mechanical design parameters must be established to effectively engineer scaffolds (biomaterials) that mimic the extracellular matrix (ECM) environment of cells and have clinical applications as tissue substitutes. In this study, three-dimensional (3-D) matrices were prepared from type I collagen, the predominant compositional and structural component of connective tissue ECMs, and structural-mechanical relationships were studied. Polymerization conditions, including collagen concentration (0.3-3 mg/mL) and pH (6-9), were varied to obtain matrices of collagen fibrils with different microstructures. Confocal reflection microscopy was used to assess specific micro-structural features (e.g., diameter and length) and organization of component fibrils in 3-D. Microstructural analyses revealed that changes in collagen concentration affected fibril density while maintaining a relatively constant fibril diameter. On the other hand, both fibril length and diameter were affected by the pH of the polymerization reaction. Mechanically, all matrices exhibited a similar stress-strain curve with identifiable "toe," "linear," and "failure" regions. However the linear modulus and failure stress increased with collagen concentration and were correlated with an increase in fibril density. Additionally, both the linear modulus and failure stress showed an increase with pH, which was related to an increasedfibril length and a decreasedfibril diameter. The tensile mechanical properties of the collagen matrices also showed strain rate dependence. Such fundamental information regarding the 3-D microstructural-mechanical properties of the ECM and its component molecules are important to our overall understanding of cell-ECM interactions (e.g., mechanotransduction) and the development of novel strategies for tissue repair and replacement.read more
Citations
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy.
Rana Rezakhaniha,Aristotelis Agianniotis,Jelle T. C. Schrauwen,Alessandra Griffa,Daniel Sage,Carlijn V. C. Bouten,F.N. van de Vosse,Michael Unser,Nikolaos Stergiopulos +8 more
TL;DR: Information on collagen fiber waviness and orientation could be used to develop structural models of the adventitia, providing better means for analyzing and understanding the mechanical properties of vascular wall.
Journal ArticleDOI
Fibroblast biology in three-dimensional collagen matrices.
TL;DR: Research on fibroblast biology in three-dimensional collagen matrices offers new opportunities to understand the reciprocal and adaptive interactions that occur between cells and surrounding matrix in a tissue-like environment.
Journal ArticleDOI
Scaffold Design for Bone Regeneration
TL;DR: This focus of this review is on the evolution of these scaffolds as bone graft substitutes in the process of recreating the bone tissue microenvironment, including biochemical and biophysical cues.
Journal ArticleDOI
Protease-dependent versus -independent cancer cell invasion programs: three-dimensional amoeboid movement revisited
TL;DR: It is demonstrated that cancer cells have an absolute requirement for the membrane-anchored metalloproteinase MT1-MMP for invasion, and that protease-independent mechanisms of cell migration are only plausible when the collagen network is devoid of the covalent cross-links that characterize normal tissues.
References
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Book
Materials Science and Engineering: An Introduction
TL;DR: In this paper, the International System of Units (SI) is used to measure the properties of materials and their properties in the context of materials science and engineering, including properties of metal alloys.
Book
Biomechanics: Mechanical Properties of Living Tissues
Yuan-Cheng Fung,Richard Skalak +1 more
TL;DR: This chapter discusses the mechanics of Erythrocytes, Leukocytes, and Other Cells, and their role in Bone and Cartilage, and the properties of Bioviscoelastic Fluids, which are a by-product of these cells.
Journal ArticleDOI
Design of an artificial skin. I. Basic design principles.
Ioannis V. Yannas,John F. Burke +1 more
TL;DR: The physiocochemical, biochemical, and mechanical considerations that form the basis for two-stage design of a membrane useful as an experimental wound closure of patients who suffer extensive loss of skin are described.
Book ChapterDOI
Preparation and characterization of the different types of collagen.
E J Miller,R K Rhodes +1 more
TL;DR: This chapter presents methods generally applicable to the preparation of native collagen from a variety of sources, conducted at relatively low temperatures, in the range of 4-8 °C, to minimizes bacterial growth, enhances the solubility of native collagens, and ensures the retention of native conformation on the part of the Solubilized Collagens.