The Activation of Type 1 and Type 2 Plasminogen by Type I and Type II Tissue Plasminogen Activator
Kazuya Mori,Raymond A. Dwek,A. Kristina Downing,Ghislain Opdenakker,Ghislain Opdenakker,Pauline M. Rudd +5 more
TLDR
In an indirect amidolytic assay involving native human Gluplasminogen and fibrin, type II tPA showed a 2-fold higher activity than type I tPA.About:
This article is published in Journal of Biological Chemistry.The article was published on 1995-02-17 and is currently open access. It has received 47 citations till now. The article focuses on the topics: Plasminogen activator inhibitor-1 & Tissue plasminogen activator.read more
Citations
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Journal ArticleDOI
Concepts and Principles of O-Linked Glycosylation
TL;DR: The biosynthesis, structures, and functions of O-glycosylation, as a complex posttranslational event, is reviewed and compared and the recent development of novel technologies for glycan analysis promises to yield new insights in the factors that determine site occupancy, structure-function relationship, and the contribution of O -linked sugars to physiological and pathological processes.
Journal ArticleDOI
Gelatinase B functions as regulator and effector in leukocyte biology
Ghislain Opdenakker,Van den Steen Pe,Bénédicte Dubois,Inge Nelissen,Van Coillie E,Stefan Masure,Paul Proost,Van Damme J +7 more
TL;DR: Gelatinase B ablation in the mouse leads to altered bone remodeling and subfertility, results in resistance to several induced inflammatory or autoimmune pathologies, and indicates that the enzyme plays a crucial role in development and angiogenesis.
Journal ArticleDOI
Glycosylation: heterogeneity and the 3D structure of proteins.
Pauline M. Rudd,Raymond A. Dwek +1 more
TL;DR: Glycoproteins generally exist as populations of glycosylated variants (glycoforms) of a single polypeptide and the recognition of identical motifs in different glycans allows a heterogeneous population of glycoforms to participate in specific biological interactions.
Journal ArticleDOI
Plasminogen activators and matrix metalloproteases, mediators of extracellular proteolysis in inflammatory demyelination of the central nervous system
M L Cuzner,Ghislain Opdenakker +1 more
TL;DR: Novel treatments for demyelinating diseases may be predicted by specific inhibition of these enzymes, as MMPs modify matrix components, promoting extravasation of lymphocytes and monocytes/macrophages and have the potential to generate encephalitogenic peptides from myelin basic protein.
Journal ArticleDOI
The X-ray crystal structure of full-length human plasminogen
Ruby H. P. Law,Tom T. Caradoc-Davies,Nathan Cowieson,Anita J Horvath,Adam J. Quek,Joanna Amarante Encarnacao,Joanna Amarante Encarnacao,David Steer,Angus Cowan,Qingwei Zhang,Bernadine G.C. Lu,Robert N. Pike,A. Ian Smith,Paul Bernard Coughlin,James C. Whisstock +14 more
TL;DR: The structure of closed plasminogen reveals that the PAp and SP domains, together with chloride ions, maintain the closed conformation through interactions with the kringle array, and analysis of the structure suggests that KR5 peeling away from thePAp domain may initiate plAsminogen conformational change.
References
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Book ChapterDOI
Plasminogen activators, tissue degradation, and cancer.
Keld Danø,Peter A. Andreasen,J Grøndahl-Hansen,Peter Marcus Kristensen,L.S. Nielsen,L. Skriver +5 more
TL;DR: This chapter describes two types of plasminogen activators—namely, the urokinase-type plasMinogen activator (u-PA) and the tissue- type plasmineg activator(t-PA), which are essentially different gene products.
Journal ArticleDOI
Purification and characterization of the plasminogen activator secreted by human melanoma cells in culture.
D C Rijken,Desire Collen +1 more
TL;DR: In immunodiffusion, as well as in quenching experiments of the fibrinolytic activities, the melanoma plasminogen activator appeared to be immunologically identical with the uterine tissue plasmineg activator, but unrelated to urokinase.
Journal ArticleDOI
Basic and clinical aspects of fibrinolysis and thrombolysis.
Desire Collen,H.R. Lijnen +1 more
TL;DR: Reduction of infarct size, preservation of ventricular function, and reduction in mortality has been obtained with SK, rt-PA, and APSAC, and thrombolytic therapy has become standard treatment for early acute myocardial infarction.
Journal ArticleDOI
A simple, sensitive spectrophotometric assay for extrinsic (tissue-type) plasminogen activator applicable to measurements in plasma.
TL;DR: An indirect spectrophotometric assay for extrinsic plasminogen activator has been devised, which is based on the parabolic assay of Drapier et al. (5), and the application for intrinsic plasmineogen activation measurements in plasma euglobulin fractions is demonstrated.
Journal ArticleDOI
Measurement of the binding of antifibrinolytic amino acids to various plasminogens
TL;DR: This method requires very small amounts of protein and allows determination of low as well as very high dissociation constants with equal reliability.