The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer.
Yupeng Qiu,Yupeng Qiu,Robert F Levendosky,Srinivas Chakravarthy,Ashok Kumar Patel,Gregory D. Bowman,Sua Myong,Sua Myong +7 more
TLDR
It is demonstrated that the monomeric Chd1 remodeler shifts DNA back and forth by dynamically alternating between different segments of the nucleosome, and proposed that active interplay of the ATPase motor with the regulatory domains may promote dynamic nucleosomes structures uniquely suited for histone exchange and chromatin reorganization during transcription.About:
This article is published in Molecular Cell.The article was published on 2017-10-05 and is currently open access. It has received 52 citations till now. The article focuses on the topics: Nucleosome assembly & Linker DNA.read more
Citations
More filters
Journal ArticleDOI
Structure and regulation of the human INO80–nucleosome complex
Rafael Ayala,Oliver Willhoft,Ricardo J. Aramayo,Martin Wilkinson,Elizabeth A. McCormack,Lorraine Ocloo,Dale B. Wigley,Xiaodong Zhang +7 more
TL;DR: The structure of the human INO 80 chromatin remodeller with a bound nucleosome is presented, which reveals that INO80 interacts with nucleosomes in a previously undescribed manner: the motor domains are located on the DNA at the entry point to the nucleosom, rather than at superhelical location 2.
Journal ArticleDOI
Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome
Ramasubramanian Sundaramoorthy,Amanda L Hughes,Hassane El-Mkami,David Norman,Helder Ferreira,Tom Owen-Hughes +5 more
TL;DR: Sundaramoorthy et al. as mentioned in this paper studied the structure of Chd1 bound to a nucleosome, in which one histone protein was modified with a molecule, called ubiquitin, which is present on genes where CHd1 is known to be active.
Journal ArticleDOI
Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling
TL;DR: It is proposed that DNA buffering ensures nucleosome stability during ATP-dependent remodelling, and provides a means for communication between remodellers acting on opposite sides of the nucleosomes in real-time.
Journal ArticleDOI
Single-Molecule Analysis and Engineering of DNA Motors
TL;DR: Techniques such as mutagenesis, chemical modifications, and optogenetics that have been used to re-engineer existing molecular motors to have, for instance, altered speed, processivity, or functionality are reviewed.
Journal ArticleDOI
RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36
TL;DR: It is demonstrated that unlike on G4-DNA, DHX36 displays ATP-independent unfolding of G4 -RNA followed by ATP-dependent refolding, generating a highly asymmetric pattern of activity.
References
More filters
Journal ArticleDOI
Uniqueness of ab initio shape determination in small-angle scattering
TL;DR: In this article, scattering patterns from geometrical bodies with different shapes and anisometry (solid and hollow spheres, cylinders, prisms) are computed and the shapes are reconstructed using envelope function and bead modelling methods.
Journal ArticleDOI
A practical guide to single-molecule FRET
TL;DR: A practical guide to using smFRET, focusing on the study of immobilized molecules that allow measurements of single-molecule reaction trajectories from 1 ms to many minutes, is provided.
Journal ArticleDOI
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
P.T Lowary,Julia R. Widom +1 more
TL;DR: In this paper, a set of highest affinity molecules were selected, cloned and sequenced, their affinities (free energies) for histone octamer in nucleosome reconstitution measured, and their ability to position nucleosomes in vitro assessed by native gel electrophoresis.
Journal ArticleDOI
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering
Daniel Franke,Dmitri I. Svergun +1 more
TL;DR: DAMMIF, an enhanced and significantly faster implementation of the ab-initio shape-determination program DAMMIN for small-angle scattering data, is presented.
Journal ArticleDOI
ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions
Daniel Franke,Maxim V. Petoukhov,Maxim V. Petoukhov,Peter V. Konarev,Peter V. Konarev,Alejandro Panjkovich,Anne T. Tuukkanen,Haydyn D. T. Mertens,Alexey Kikhney,Nelly R. Hajizadeh,J.M. Franklin,Cy M. Jeffries,Dmitri I. Svergun +12 more
TL;DR: Developments and improvements of the ATSAS software suite for analysis of small-angle scattering data of biological macromolecules or nanoparticles are described.