Journal ArticleDOI
The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes
Ophelia Papoulas,Shelley J. Beek,Sarah L. Moseley,Claire M. McCallum,Melinda Sarte,Allen Shearn,John W. Tamkun +6 more
TLDR
The trithorax group gene brahma (brm) encodes an activator of Drosophila homeotic genes that functions as the ATPase subunit of a large protein complex as discussed by the authors.Abstract:
The trithorax group gene brahma (brm) encodes an activator of Drosophila homeotic genes that functions as the ATPase subunit of a large protein complex. To determine if BRM physically interacts with other trithorax group proteins, we purified the BRM complex from Drosophila embryos and analyzed its subunit composition. The BRM complex contains at least seven major polypeptides. Surprisingly, the majority of the subunits of the BRM complex are not encoded by trithorax group genes. Furthermore, a screen for enhancers of a dominant-negative brm mutation identified only one trithorax group gene, moira (mor), that appears to be essential for brm function in vivo. Four of the subunits of the BRM complex are related to subunits of the yeast chromatin remodeling complexes SWI/SNF and RSC. The BRM complex is even more highly related to the human BRG1 and hBRM complexes, but lacks the subunit heterogeneity characteristic of these complexes. We present biochemical evidence for the existence of two additional complexes containing trithorax group proteins: a 2 MDa ASH1 complex and a 500 kDa ASH2 complex. These findings suggest that BRM plays a role in chromatin remodeling that is distinct from the function of most other trithorax group proteins.read more
Citations
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CBP/p300 in cell growth, transformation, and development
TL;DR: This review focuses on the involvement of CBP/p300 in the complex biological processes that affect cell growth, transformation, and development.
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Histone methyltransferase activity of a Drosophila Polycomb group repressor complex
Jürg Müller,Craig M. Hart,Nicole J. Francis,Marcus L. Vargas,Aditya K. Sengupta,Aditya K. Sengupta,Brigitte Wild,Ellen L. Miller,Michael B. O'Connor,Michael B. O'Connor,Robert E. Kingston,Jeffrey A. Simon +11 more
TL;DR: This work purified an ESC-E(Z) complex from Drosophila embryos and found four major subunits: ESC, E(Z), NURF-55, and the PcG repressor, SU( Z)12, which methylates lysine-27 of histone H3.
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Tight junction proteins.
TL;DR: Advances in the knowledge of the molecular structure of TJ support previous physiological models that exhibited TJ as dynamic structures that present distinct permeability and morphological characteristics in different tissues and in response to changing natural, pathological or experimental conditions.
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SWI/SNF nucleosome remodellers and cancer
TL;DR: The contributions of SWI/SNF mutations to cancer formation are discussed, their normal functions are examined and opportunities for novel therapeutic interventions for SWI /SNF-mutant cancers are discussed.
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Epigenetic Regulation of Cellular Memory by the Polycomb and Trithorax Group Proteins
Leonie Ringrose,Renato Paro +1 more
TL;DR: Current ideas on the protein and DNA components of this transcriptional memory system are reviewed and how they interact dynamically with each other to orchestrate cellular memory for several hundred genes are reviewed.
References
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TL;DR: The computer program BLASTX performed conceptual translation of a nucleotide query sequence followed by a protein database search in one programmatic step and was characterized as appropriate for use in moderate and large scale sequencing projects at the earliest opportunity, when the data are most prone to containing errors.
Journal ArticleDOI
brahma: a regulator of Drosophila homeotic genes structurally related to the yeast transcriptional activator SNF2/SWI2.
John W. Tamkun,Renate Deuring,Matthew P. Scott,Mark Kissinger,Angela M. Pattatucci,Thomas C. Kaufman,James A. Kennison,James A. Kennison,James A. Kennison +8 more
TL;DR: The brahma (brm) gene encodes a 1638 residue protein that is similar to SNF2/SWI2, a protein involved in transcriptional activation in yeast, suggesting possible models for the role of brm in the transcriptionalactivation of homeotic genes.
Journal ArticleDOI
Stimulation of GAL4 Derivative Binding to Nucleosomal DNA by the Yeast SWI/SNF Complex
TL;DR: It is shown that the purified SWI/SNF complex is composed of 10 subunits and includes the SWI1, SWI2/ SNF2, SWi3, SNF5, and SNF6 gene products, and suggests that a primary role of the SWi/SNf complex is to promote activator binding to nucleosomal DNA.