The enzymes of the galactose operon in escherichia coli. i. purification and characterization of uridine diphosphogalactose 4-epimerase.
David Wilson,David S. Hogness +1 more
TLDR
The results indicate that this galactokinase consists of one polypeptide chain containing 368 residues, and hence that its structural gene contains about 1100 base pairs.About:
This article is published in Journal of Biological Chemistry.The article was published on 1969-04-01 and is currently open access. It has received 201 citations till now. The article focuses on the topics: gal operon & Structural gene.read more
Citations
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Journal ArticleDOI
The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose
TL;DR: The structure of the enzyme reveals many details of the binding of NAD+ and inhibitors at the active site, and appears to have been guided by the principle of economy in the evolution of binding sites.
Journal ArticleDOI
Half-of-the-sites reactivity and the conformational states of cytidine triphosphate synthetase.
Book ChapterDOI
Molecular genetics of nucleotide sugar interconversion pathways in plants.
Wolf-Dieter Reiter,Gary Vanzin +1 more
TL;DR: The genetic characterization of nucleotide sugar interconversion enzymes has the potential to understand the regulation of these complex metabolic pathways and to permit the modification of cell wall material by changing the availability of monosaccharide precursors.
Journal ArticleDOI
A quantitative approach to catabolite repression in Escherichia coli.
Katja Bettenbrock,Sophia Fischer,Andreas Kremling,Knut Jahreis,Thomas Sauter,Ernst Dieter Gilles +5 more
TL;DR: The different phenomena affecting the phosphorylation level of EIIACrr, the key regulation molecule for inducer exclusion and catabolite repression in enteric bacteria, can now be explained quantitatively.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
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Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
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On the Nature of Allosteric Transitions: A Plausible Model
TL;DR: "It is certain that all bodies whatsoever, though they have no sense, yet they have perception, and whether the body be alterant or alterec, evermore a perception precedeth operation; for else all bodies would be like one to another."
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A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.
Related Papers (5)
Purification and properties of uridine diphosphate galactose 4-epimerase from yeast.
Robert A. Darrow,Robert Rodstrom +1 more
The Enzymes of the Galactose Operon in Escherichia coli II. THE SUBUNITS OF URIDINE DIPHOSPHOGALACTOSE 4-EPIMERASE
David Wilson,David S. Hogness +1 more