Journal ArticleDOI
The FT/TFL1 gene family in grapevine
TLDR
Overexpression of VvFT in transgenic Arabidopsis plants generates early flowering phenotypes similar to those produced by FT supporting a role for this gene in flowering promotion, which is consistent with the biological roles assigned to similar genes in other species.Abstract:
The FT/TFL1 gene family encodes proteins with similarity to phosphatidylethanolamine binding proteins which function as flowering promoters and repressors. We show here that the FT/TFL1 gene family in Vitis vinifera is composed of at least five genes. Sequence comparisons with homologous genes identified in other dicot species group them in three major clades, the FT, MFT and TFL1 subfamilies, the latter including three of the Vitis sequences. Gene expression patterns are in agreement with a role of VvFT and VvMFT as flowering promoters; while VvTFL1A, VvTFL1B and VvTFL1C could be associated with vegetative development and maintenance of meristem indetermination. Overexpression of VvFT in transgenic Arabidopsis plants generates early flowering phenotypes similar to those produced by FT supporting a role for this gene in flowering promotion. Overexpression of VvTFL1A does not affect flowering time but the determination of flower meristems, strongly altering inflorescence structure, which is consistent with the biological roles assigned to similar genes in other species.read more
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A Map-Based Cloning Strategy Employing a Residual Heterozygous Line Reveals that the GIGANTEA Gene Is Involved in Soybean Maturity and Flowering
Satoshi Watanabe,Zhengjun Xia,Zhengjun Xia,Rumiko Hideshima,Yasutaka Tsubokura,Shusei Sato,Naoki Yamanaka,Ryoji Takahashi,Toyoaki Anai,Satoshi Tabata,Keisuke Kitamura,Kyuya Harada +11 more
TL;DR: Results indicate that Gm GIa is the gene responsible for the E2 locus and that a null mutation in GmGIa may contribute to the geographic adaptation of soybean.
Journal ArticleDOI
The Grapevine Expression Atlas Reveals a Deep Transcriptome Shift Driving the Entire Plant into a Maturation Program
Marianna Fasoli,Silvia Dal Santo,Sara Zenoni,Giovanni Battista Tornielli,Lorenzo Farina,Anita Zamboni,Andrea Porceddu,Luca Venturini,Manuele Bicego,Vittorio Murino,Alberto Ferrarini,Massimo Delledonne,Mario Pezzotti +12 more
TL;DR: It is found that the whole plant undergoes transcriptomic reprogramming, driving it towards maturity, which may be a defining characteristic of perennial woody plants.
Journal ArticleDOI
MOTHER OF FT AND TFL1 Regulates Seed Germination through a Negative Feedback Loop Modulating ABA Signaling in Arabidopsis
TL;DR: It is demonstrated here that MOTHER of FT and TFL1 (MFT), which encodes a phosphatidylethanolamine-binding protein, regulates seed germination via the ABA and GA signaling pathways in Arabidopsis thaliana.
Journal ArticleDOI
The multifaceted roles of FLOWERING LOCUS T in plant development
Pierre A. Pin,Ove Nilsson +1 more
TL;DR: The plethora of roles that FT-like genes have acquired during evolution are assessed and their implications in plant diversity, adaptation and domestication are assessed.
Journal ArticleDOI
The FLOWERING LOCUS T/TERMINAL FLOWER 1 Gene Family: Functional Evolution and Molecular Mechanisms
TL;DR: The comprehensively survey the conserved and diverse functions of the FT/TFL1 gene family throughout the plant kingdom, summarize new findings regarding the unique evolution of FT in multiple species, and highlight recent work elucidating the molecular mechanisms of these proteins.
References
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Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
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TL;DR: An overview of the statistical methods, computational tools, and visual exploration modules for data input and the results obtainable in MEGA is provided.
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George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Journal ArticleDOI
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer
TL;DR: The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases.