The Host Antimicrobial Protein Calgranulin C Participates in the Control of Campylobacter jejuni Growth via Zinc Sequestration.
Janette M. Shank,Brittni R. Kelley,Joseph W. Jackson,Jessica L. Tweedie,Dana Franklin,Steven M. Damo,Jennifer A. Gaddy,Jennifer A. Gaddy,Caitlin N. Murphy,Jeremiah G. Johnson +9 more
TLDR
Results suggest that upon infection with C. jejuni, leukocytes trafficked to the intestine release calgranulin C as a mechanism for inhibiting C.Jejuni growth.Abstract:
Campylobacter jejuni is a leading cause of bacterially derived gastroenteritis worldwide. Campylobacter is most commonly acquired through the consumption of undercooked poultry meat or through drinking contaminated water. Following ingestion, Campylobacter adheres to the intestinal epithelium and mucus layer, causing toxin-mediated inflammation and inhibition of fluid reabsorption. Currently, the human response to infection is relatively unknown, and animal hosts that model these responses are rare. As such, we examined patient fecal samples for the accumulation of the neutrophil protein calgranulin C during infection with Campylobacter jejuni In response to infection, calgranulin C was significantly increased in the feces of humans. To determine whether calgranulin C accumulation occurs in an animal model, we examined disease in ferrets. Ferrets were effectively infected by C. jejuni, with peak fecal loads observed at day 3 postinfection and full resolution by day 12. Serum levels of interleukin-10 (IL-10) and tumor necrosis factor alpha (TNF-α) significantly increased in response to infection, which resulted in leukocyte trafficking to the colon. As a result, calgranulin C increased in the feces of ferrets at the time when C. jejuni loads decreased. Further, the addition of purified calgranulin C to C. jejuni cultures was found to inhibit growth in a zinc-dependent manner. These results suggest that upon infection with C. jejuni, leukocytes trafficked to the intestine release calgranulin C as a mechanism for inhibiting C. jejuni growth.read more
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Journal ArticleDOI
Nutrient Zinc at the Host-Pathogen Interface.
TL;DR: The mechanisms of vertebrate and bacterial zinc homeostasis and mobilization are discussed, as well as recent developments in the understanding of microbial zinc acquisition.
Book ChapterDOI
S100 Proteins in the Innate Immune Response to Pathogens.
Natalia Kozlyuk,Andrew J. Monteith,Velia Garcia,Steven M. Damo,Steven M. Damo,Eric P. Skaar,Walter J. Chazin +6 more
TL;DR: Protocols for these S100 proteins for high-level production of recombinant protein, measurement of binding affinities using isothermal titration calorimetry, and an assay of antimicrobial activity are presented.
Journal ArticleDOI
S100A12 in Digestive Diseases and Health: A Scoping Review
Alexandre Carvalho,Jacky Lu,Jamisha D. Francis,Rebecca E. Moore,Kathryn P. Haley,Ryan S. Doster,Ryan S. Doster,Steven D. Townsend,Jeremiah G. Johnson,Steven M. Damo,Steven M. Damo,Jennifer A. Gaddy,Jennifer A. Gaddy,Jennifer A. Gaddy +13 more
TL;DR: Results suggest that S100A12 is implicated in gastroenteritis, necrotizing enterocolitis, gastritis, gastric cancer, Crohn's disease, irritable bowel syndrome, inflammatory bowel disease, and digestive tract cancers.
Journal ArticleDOI
RNA Sequencing Reveals Distinct Immune Responses in the Chorioamniotic Membranes of Women with Preterm Labor and Microbial or Sterile Intra-amniotic Inflammation.
Kenichiro Motomura,Kenichiro Motomura,Roberto Romero,Jose Galaz,Jose Galaz,Adi L. Tarca,Adi L. Tarca,Adi L. Tarca,Bogdan Done,Bogdan Done,Yi Xu,Yi Xu,Yaozhu Leng,Yaozhu Leng,Valeria Garcia-Flores,Valeria Garcia-Flores,Marcia Arenas-Hernandez,Marcia Arenas-Hernandez,Kevin R. Theis,Kevin R. Theis,Meyer Gershater,Meyer Gershater,Eun Jung Jung,Eun Jung Jung,Chaur-Dong Hsu,Chaur-Dong Hsu,Nardhy Gomez-Lopez,Nardhy Gomez-Lopez +27 more
TL;DR: In this paper, the authors found that there were significant transcriptomic differences involving host response to pathogens in the chorioamniotic membranes of women with intra-amniotic infection compared to those from women without inflammation.
Journal ArticleDOI
Induction of neutrophil extracellular traps by Campylobacter jejuni.
Sean M. Callahan,Ryan S. Doster,Joseph W. Jackson,Joseph W. Jackson,Brittni R. Kelley,Jennifer A. Gaddy,Jeremiah G. Johnson +6 more
TL;DR: It is found that purified human neutrophils are activated by C. jejuni and exhibit signatures of NET generation, including presence of protein arginine deiminase‐4, histone citrullination, myeloperoxidase, neutrophil elastase release and DNA extrusion, which provide further understanding of C.Jejuni–neutrophil interactions and inflammatory responses during campylobacteriosis.
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