Journal ArticleDOI
The intron of the mitochondrial 21S rRNA gene: distribution in different yeast species and sequence comparison between Kluyveromyces thermotolerans and Saccharomyces cerevisiae.
Alain Jacquier,Bernard Dujon +1 more
TLDR
Comparison of the two yeast r1-introns with equivalent introns of N. crassa and A. nidulans mitochondria reveals that introns with very similar RNA secondary structures can accommodate different types of ORFs.Abstract:
We have screened numerous different yeast species for the presence of sequences homologous to the intron of the mitochondrial 21S rRNA gene of Saccharomyces cerevisiae (intron r1) and found them in all Kluyveromyces species, some of the Saccharomyces species and none of the other yeasts tested. We have determined the nucleotide sequence of the r1-intron in K. thermotolerans and compared it with that of S. cerevisiae. The two introns are inserted at the same position within the 21S rRNA gene. They contain homologous internal open reading frames (ORFs) initiated at the same AUG codon which can be aligned over their entire length. Several silent multi-substitutions indicate that these intronic ORFs represent selectively conserved functional genes. Other intron segments, on the contrary, reveal short blocks of extensive homology separated by non-homologous stretches and/or additions-deletions. Comparison of our two yeast r1-introns with equivalent introns of N. crassa and A. nidulans mitochondria reveals that introns with very similar RNA secondary structures can accommodate different types of ORFs.read more
Citations
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Journal ArticleDOI
Food fermentations: Microorganisms with technological beneficial use
F Bourdichon,Serge Casaregola,Choreh Farrokh,Jens Christian Frisvad,Monica L. Gerds,Walter P. Hammes,James Harnett,Geert Huys,Svend Laulund,Arthur C. Ouwehand,Ian B. Powell,Jashbhai B. Prajapati,Yasuyuki Seto,Eelko Ter Schure,Aart Van Boven,Vanessa Vankerckhoven,Annabelle Zgoda,Sandra Tuijtelaars,Egon Bech Hansen +18 more
TL;DR: An updated inventory of microorganisms used in food fermentations covering a wide range of food matrices and the taxonomy is reviewed and updated in order to bring theTaxonomy in agreement with the current standing in nomenclature.
Journal ArticleDOI
Group I introns as mobile genetic elements: facts and mechanistic speculations--a review.
TL;DR: Mechanisms of intron acquisition and intron loss must create an equilibrium, which explains the irregular distribution of group I introns in various genetic systems and predicts that horizontal transfer ofintron sequences must occur between unrelated species, using vectors yet to be discovered.
Journal ArticleDOI
An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene
Alain Jacquier,Bernard Dujon +1 more
TL;DR: It is shown that ORF encodes a protein active in the gene conversion that spreads the intron within populations of interbreeding strains and is reminiscent of the "transposase" encoded by mobile genetic elements and is discussed in relation to other intron functions.
Journal ArticleDOI
Recurrent invasion and extinction of a selfish gene
Matthew R. Goddard,Austin Burt +1 more
TL;DR: The data support a cyclical model of invasion, degeneration, and loss, followed by reinvasion, and each of these transitions is estimated to occur about once every 2 million years, consistent with the idea that frequent horizontal transmission is necessary for the long-term persistence of homing endonuclease genes.
Journal ArticleDOI
Universal code equivalent of a yeast mitochondrial intron reading frame is expressed into E. coli as a specific double strand endonuclease
Laurence Colleaux,L. d'Auriol,M. Betermier,G. Cottarel,Alain Jacquier,F. Galibert,Bernard Dujon +6 more
TL;DR: A universal code equivalent of the r1 ORF is constructed that, under appropriate promoter control, allows the overexpression in E. coli of a protein identical to the mitochondrial intron encoded "transposase", which exhibits a double strand endonuclease activity specific for the omega- site.
References
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Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
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Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
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Journal ArticleDOI
Organization and Expression of Eucaryotic Split Genes Coding for Proteins
R Breathnach,P Chambon +1 more
TL;DR: This paper organizes the organization of protein codes into split genes, a small number of which are expressed in the chickenuct, and discusses generalization, generalization and Molecular Evolution.
Expression of eucaryotic split genes coding for proteins
TL;DR: In this paper, the split genes were described as follows: Globin genes expressed in the chicken o,iduct, Vitellogenin genes, Collagen genes and Actin genes.