The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells.
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TLDR
This review will examine the methods through which bioprinted stem cells are differentiated into desired cell lineages through biochemical, biological, and biomechanical techniques.Abstract:
The field of tissue engineering and regenerative medicine has made numerous advances in recent years in the arena of fabricating multifunctional, three-dimensional (3D) tissue constructs This can be attributed to novel approaches in the bioprinting of stem cells There are expansive options in bioprinting technology that have become more refined and specialized over the years, and stem cells address many limitations in cell source, expansion, and development of bioengineered tissue constructs While bioprinted stem cells present an opportunity to replicate physiological microenvironments with precision, the future of this practice relies heavily on the optimization of the cellular microenvironment To fabricate tissue constructs that are useful in replicating physiological conditions in laboratory settings, or in preparation for transplantation to a living host, the microenvironment must mimic conditions that allow bioprinted stem cells to proliferate, differentiate, and migrate The advances of bioprinting stem cells and directing cell fate have the potential to provide feasible and translatable approach to creating complex tissues and organs This review will examine the methods through which bioprinted stem cells are differentiated into desired cell lineages through biochemical, biological, and biomechanical techniquesread more
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A photo-crosslinkable cartilage-derived extracellular matrix bioink for auricular cartilage tissue engineering.
Dafydd O. Visscher,Hyeongjin Lee,Paul P. M. van Zuijlen,Marco N. Helder,Anthony Atala,James J. Yoo,Sang Jin Lee +6 more
TL;DR: The results showed cdCEM was obtained with complete removal of cellular components while preserving major ECM proteins, and the potential of cell-based bioprinting using this cartilage-specific dECMMA bioink is demonstrated as an alternative option for auricular cartilage reconstruction.
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DNA-Based Dynamic Mimicry of Membrane Proteins for Programming Adaptive Cellular Interactions.
Jin Li,Jin Li,Kanyu Xun,Liyan Zheng,Xueyu Peng,Liping Qiu,Weihong Tan,Weihong Tan,Weihong Tan +8 more
TL;DR: In this article, a cell-surface nano-architecture that realizes molecular-recognition-initiated DNA assembly to mimic the dynamic behavior of membrane proteins, enabling the manipulation of cellular interaction in response to environmental changes.
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Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles.
Yijie Zhang,Enhejirigala,Bin Yao,Bin Yao,Zhao Li,Wei Song,Jianjun Li,Dongzhen Zhu,Yuzhen Wang,Xianlan Duan,Xianlan Duan,Xingyu Yuan,Xingyu Yuan,Sha Huang,Xiaobing Fu,Xiaobing Fu +15 more
TL;DR: In this article, a combined model was created by seeding hair follicles on 3D printed sweat glands and hair spheroids, and the interaction between SG scaffolds and HF spheroid was detected using RNA expression and immunofluorescence staining.
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Bioprinting and regeneration of auricular cartilage using a bioactive bioink based on microporous photocrosslinkable acellular cartilage matrix
Litao Jia,Yujie Hua,Jinshi Zeng,Wenshuai Liu,Di Wang,Guang Sen Zhou,Xia Liu,Haiyue team Jiang +7 more
TL;DR: In this article , a biomimetic microporous methacrylate-modified acellular cartilage matrix (ACMMA) was used for the development of biological auricle equivalents with precise shapes, low immunogenicity, and excellent mechanics using auricular chondrocytes.
References
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Hydrogels with Time-Dependent Material Properties Enhance Cardiomyocyte Differentiation In Vitro
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