The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice
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TLDR
The results show that SAPK4 regulates ion homeostasis and growth and development under salinity and suggest function of SAPK 4 as a regulatory factor in plant salt stress acclimation and identification of signaling elements involved in stress adaptation in plants is suggested.Abstract:
Plants respond to extracellularly perceived abiotic stresses such as low temperature, drought, and salinity by activation of complex intracellular signaling cascades that regulate acclimatory biochemical and physiological changes. Protein kinases are major signal transduction factors that have a central role in mediating acclimation to environmental changes in eukaryotic organisms. In this study, we characterized the function of the sucrose nonfermenting 1-related protein kinase2 (SnRK2) SAPK4 in the salt stress response of rice. Translational fusion of SAPK4 with the green fluorescent protein (GFP) showed subcellular localization in cytoplasm and nucleus. To examine the role of SAPK4 in salt tolerance we generated transgenic rice plants with over-expression of rice SAPK4 under control of the CaMV-35S promoter. Induced expression of SAPK4 resulted in improved germination, growth and development under salt stress both in seedlings and mature plants. In response to salt stress, the SAPK4-overexpressing rice accumulated less Na+ and Cl- and showed improved photosynthesis. SAPK4-regulated genes with functions in ion homeostasis and oxidative stress response were identified: the vacuolar H+-ATPase, the Na+/H+ antiporter NHX1, the Cl- channel OsCLC1 and a catalase. Our results show that SAPK4 regulates ion homeostasis and growth and development under salinity and suggest function of SAPK4 as a regulatory factor in plant salt stress acclimation. Identification of signaling elements involved in stress adaptation in plants presents a powerful approach to identify transcriptional activators of adaptive mechanisms to environmental changes that have the potential to improve tolerance in crop plants.read more
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Gene Expression Profiling of Plants under Salt Stress
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References
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Journal ArticleDOI
Oxidative stress, antioxidants and stress tolerance
TL;DR: Key steps of the signal transduction pathway that senses ROIs in plants have been identified and raise several intriguing questions about the relationships between ROI signaling, ROI stress and the production and scavenging ofROIs in the different cellular compartments.
Journal ArticleDOI
THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons
TL;DR: Whenever the water-water cycle operates properly for scavenging of active oxygens in chloroplasts, it also effectively dissipates excess excitation energy under environmental stress.
Journal ArticleDOI
OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought‐, high‐salt‐ and cold‐responsive gene expression
Joseph G. Dubouzet,Yoh Sakuma,Yusuke Ito,Mie Kasuga,Emilyn G. Dubouzet,Setsuko Miura,Motoaki Seki,Kazuo Shinozaki,Kazuko Yamaguchi-Shinozaki +8 more
TL;DR: OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses and has functional similarity to DREB 1A, however, in microarray and RNA blot analyses, some stress-inducible target genes of the DREb1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDRE B1A transgenic Arabidopsis.
Journal ArticleDOI
Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses
TL;DR: The regulated overexpression of Escherichia coli trehalose biosynthetic genes (otsA and otsB) as a fusion gene for manipulating abiotic stress tolerance in rice demonstrates the feasibility of engineering rice for increased tolerance of abiotics stress and enhanced productivity through tissue-specific or stress-dependent overproduction of trehalOSE.
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Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants.
Hilde Willekens,Sangpen Chamnongpol,Mark W. Davey,Martina Schraudner,Christian Langebartels,Marc Van Montagu,Dirk Inzé,Wim Van Camp +7 more
TL;DR: Leaf necrosis correlated with accumulation of oxidized glutathione and a 4‐fold decrease in ascorbate, indicating that catalase is critical for maintaining the redox balance during oxidative stress and may not be limited to peroxisomal H2O2 production.