Journal ArticleDOI
The Sry-related gene Sox9 is expressed during chondrogenesis in mouse embryos
Edwina Wright,Murray Hargrave,Jeffrey H. Christiansen,Leanne Cooper,Jutta Kun,Timothy Evans,Uma Gangadharan,Andy Greenfield,Peter Koopman +8 more
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TLDR
The expression pattern and chromosomal location of Sox9 suggest that it may be the gene defective in the mouse skeletal mutant Tail–short, a potential animal model for campomelic dysplasia.Citations
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Sox9 is required for cartilage formation.
TL;DR: The results identify Sox9 as the first transcription factor that is essential for chondrocyte differentiation and cartilage formation and Sox9 is identified as a regulator of the chondROcyte lineage.
Journal ArticleDOI
Optical Projection Tomography as a Tool for 3D Microscopy and Gene Expression Studies
James Sharpe,Ulf Ahlgren,Paul Perry,Bill Hill,Allyson Ross,Jacob Hecksher-Sørensen,Richard Baldock,Duncan Davidson +7 more
TL;DR: This work has developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters.
Journal ArticleDOI
SOX9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1(II) collagen gene.
TL;DR: The results strongly suggest a model whereby SOX9 is involved in the control of the cell-specific activation of COL2A1 in chondrocytes, an essential component of the differentiation program of these cells.
Journal ArticleDOI
Phylogeny of the SOX family of developmental transcription factors based on sequence and structural indicators.
TL;DR: A robust phylogeny of SOX genes is proposed that reflects their evolutionary history in metazoans and finds support for subdivision of the family into groups A-H, as has been suggested in some previous studies, and for the assignment of two new groups, I and J.
Journal ArticleDOI
SOX9 directly regulates the type-II collagen gene.
Donald M. Bell,Keith K.H. Leung,Susan C. Wheatley,Ling Jim Ng,Sheila Zhou,Kam Wing Ling,Mai Har Sham,Peter Koopman,Peter Koopman,Patrick P.L. Tam,Kathryn S.E. Cheah +10 more
TL;DR: It is shown that SOX9 protein binds specifically to sequences in the first intron of human COL2A1, the gene encoding type-ll collagen, which implicate abnormal regulation of COL2a1 during chondrogenesis as a cause of the skeletal abnormalities associated with campomelic dysplasia.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
The scanning model for translation: an update.
TL;DR: The small (40S) subunit of eukaryotic ribosomes is believed to bind initially at the capped 5'-end of messenger RNA and then migrate, stopping at the first AUG codon in a favorable context for initiating translation.
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Expression of a single transfected cDNA converts fibroblasts to myoblasts.
TL;DR: In this article, the major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family, and the expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts.
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Male development of chromosomally female mice transgenic for Sry
TL;DR: It is shown that Sry on a 14-kilobase genomic DNA fragment is sufficient to induce testis differentiation and subsequent male development when introduced into chromosomally female mouse embryos.
Journal ArticleDOI
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes
John Gubbay,Jérôme Collignon,Peter Koopman,Blanche Capel,Androulla Economou,Andrea Münsterberg,Nigel Vivian,Peter N. Goodfellow,Robin Lovell-Badge +8 more
TL;DR: A gene mapping to the sex-determining region of the mouse Y chromosome is deleted in a line of XY female mice mutant for Tdy, and is expressed at a stage during male gonadal development consistent with its having a role in testis determination.