The Structure and Function of DNA G-Quadruplexes.
Jochen Spiegel,Santosh Adhikari,Shankar Balasubramanian +2 more
- Vol. 2, Iss: 2, pp 123-136
TLDR
A perspective on the structure and function of G4s is provided with an emphasis on key molecules and methodological advances that enable the study of G 4 structures in human cells and critically examine recent mechanistic insights into G4 biology and protein interaction partners.Abstract:
Guanine-rich DNA sequences can fold into four-stranded, noncanonical secondary structures called G-quadruplexes (G4s). G4s were initially considered a structural curiosity, but recent evidence suggests their involvement in key genome functions such as transcription, replication, genome stability, and epigenetic regulation, together with numerous connections to cancer biology. Collectively, these advances have stimulated research probing G4 mechanisms and consequent opportunities for therapeutic intervention. Here, we provide a perspective on the structure and function of G4s with an emphasis on key molecules and methodological advances that enable the study of G4 structures in human cells. We also critically examine recent mechanistic insights into G4 biology and protein interaction partners and highlight opportunities for drug discovery.read more
Citations
More filters
Journal ArticleDOI
G-quadruplexes: a promising target for cancer therapy.
TL;DR: In this article, a correlation between G4 structure formation and an increased intratumor heterogeneity was identified, which suggests that G4 structures might allow breast cancer stratification and support the identification of new personalized treatment options.
Journal ArticleDOI
G-quadruplex, Friend or Foe: The Role of the G-quartet in Anticancer Strategies.
TL;DR: The latest achievements and breakthroughs in the use of G4 nucleic acids as both therapeutic tools and targets for anticancer drugs and targets, particularly using aptamers and quadruplex-targeted ligands, respectively are summarized.
Journal ArticleDOI
Recent progress in non-native nucleic acid modifications.
TL;DR: A review of non-native modifications and the challenges faced in the design, synthesis, application and outlook of novel modified oligonucleotides can be found in this article, where the authors provide an overview of nonnative modifications.
Journal ArticleDOI
Molecular photoswitches in aqueous environments
TL;DR: Water-solubility of photoswitchable organic molecules has been studied in this article, where the authors focus on fully water-soluble photoswitches, such as those used in biological environments, in both in vitro and in vivo studies.
References
More filters
Journal ArticleDOI
Hallmarks of cancer: the next generation.
TL;DR: Recognition of the widespread applicability of these concepts will increasingly affect the development of new means to treat human cancer.
Journal ArticleDOI
Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid
James D. Watson,Francis Crick +1 more
TL;DR: The determination in 1953 of the structure of deoxyribonucleic acid (DNA), with its two entwined helices and paired organic bases, was a tour de force in X-ray crystallography and opened the way for a deeper understanding of perhaps the most important biological process.
Journal ArticleDOI
Quadruplex DNA: sequence, topology and structure
TL;DR: This survey focuses on the folding and structural features on quadruplexes formed from telomeric and non-telomeric DNA sequences, and examines fundamental aspects of topology and the emerging relationships with sequence.
Journal ArticleDOI
Direct evidence for a G-quadruplex in a promoter region and its targeting with a small molecule to repress c-MYC transcription.
TL;DR: The principle that c-MYC transcription can be controlled by ligand-mediated G-quadruplex stabilization is established, establishing the principle that the purine-rich strand of the DNA in this region can form two different intramolecular G- quadruplex structures.
Journal ArticleDOI
Crystal structure of parallel quadruplexes from human telomeric DNA.
TL;DR: This crystal structure of a quadruplex formed from four consecutive human telomeric DNA repeats and grown at a K+ concentration that approximates its intracellular concentration suggests a straightforward path for telomere folding and unfolding, as well as ways in which it can recognize telomerre-associated proteins.