Transbilayer movement of dipalmitoylphosphatidylcholine in proteoliposomes reconstituted from detergent extracts of endoplasmic reticulum. Kinetics of transbilayer transport mediated by a single flippase and identification of protein fractions enriched in flippase activity.
TLDR
An assay to measure the transbilayer translocation of dipalmitoylphosphatidylcholine, a membrane-embedded phospholipid, in proteoliposomes generated from detergent-solubilized rat liver endoplasmic reticulum is described.About:
This article is published in Journal of Biological Chemistry.The article was published on 2002-07-12 and is currently open access. It has received 56 citations till now. The article focuses on the topics: Phospholipid translocation & Flippase.read more
Citations
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Journal ArticleDOI
Regulation of transbilayer plasma membrane phospholipid asymmetry.
TL;DR: It is shown that the flippase is highly selective for phosphatidylserine and functions to keep this lipid sequestered from the cell surface, and the ABC class of transmembrane transporters, at least two members of this class display selectivity for their substrate lipid.
Journal ArticleDOI
Lipid flippases and their biological functions.
TL;DR: This review summarizes recent progress on the identification and characterization of the various flippases and the demonstration of their biological functions.
Journal ArticleDOI
The reconstituted P-glycoprotein multidrug transporter is a flippase for glucosylceramide and other simple glycosphingolipids
TL;DR: It is concluded that reconstituted Pgp functions as a broad-specificity outwardly-directed flippase for simple glycosphingolipids and membrane phospholIPids.
Journal ArticleDOI
Flipping lipids: why an' what's the reason for?
Sumana Sanyal,Anant K. Menon +1 more
TL;DR: Recent progress on this fundamental topic is summarized and the mechanism(s) by which biogenic membrane flippases facilitate transbilayer lipid movement is speculated.
Journal ArticleDOI
An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli.
TL;DR: The results implicate pmrL and pmrM, but not arnT, in transporting undecaprenyl phosphate-α-l-Ara4N across the inner membrane.
References
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A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
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A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids
D. Wessel,U.I. Flügge +1 more
TL;DR: A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed.
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Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots
TL;DR: Separation of polar lipids by two-dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described.
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Physics of chemoreception
Howard C. Berg,Edward M. Purcell +1 more
TL;DR: The chemotactic sensitivity of Escherichia coli approaches that of the cell of optimum design, and data on bacteriophage absorption, bacterial chemotaxis, and chemoattractant in a cellular slime mold are evaluated.
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