Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum
Hoang V. Tang,D. R. Pring,Lynn C. Shaw,Reggie A. Salazar,Figuhr R. Muza,Bin Yan,K. F. Schertz +6 more
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TLDR
Novel observations indicate that mitochondrial open reading frames associated with cms in different species can include highly similar motifs, and that fertility restoration could involve a mechanism by which synthesis of a cms-associated gene product may be precluded through internal transcript cleavage.Abstract:
Summary
A chimeric mitochondrial DNA (mtDNA) configuration of the cytoplasmic male-sterile (cms) sorghum line IS1112C includes a 321 bp open reading frame designated orf107, encoding a predicted polypeptide product of 11.85 kDa. The open reading frame, similar to several other genes associated with cms, consists of amino-terminal sequences derived from an obligate gene. Unlike other examples to date, however, the carboxy-terminal sequences are highly similar to the carboxy terminus of an open reading frame implicated in cms of rice, orf79. The amino-terminal 31 residues of orf107 are 84% similar to atp9, and the carboxy-terminal 49 residues are 57% identical and 80% similar to the carboxy terminus of orf79. Transcripts of orf107 are edited, with four C-to-U changes that alter amino acids. Sorghum lines partially or fully restored to fertility exhibit a high-efficiency internal-orf107 transcript processing activity, precluding abundant whole-length transcripts, while male-sterile lines exhibit only a trace of the activity. Previous data on the abundance of a 12 kDa in organello-synthesized polypeptide in male-sterile versus male-fertile lines are correlated with differential orf107 transcript processing activity of these lines. Examinations of backcross and F2 lines suggest a gametophytic mode of restoration, and indicate that enhanced transcript processing activity is necessary, but not sufficient, to restore full fertility. These novel observations indicate that mitochondrial open reading frames associated with cms in different species can include highly similar motifs, and that fertility restoration could involve a mechanism by which synthesis of a cms-associated gene product may be precluded through internal transcript cleavage.read more
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Journal ArticleDOI
Interactions of Mitochondrial and Nuclear Genes That Affect Male Gametophyte Development
TL;DR: Apart from their agronomic importance in hybrid seed production, mutations that encode cytoplasmic male sterility provide a means to probe the role of the mitochondrion in reproductive development.
Journal ArticleDOI
The molecular basis of cytoplasmic male sterility and fertility restoration
TL;DR: There are now several well-characterized CMS systems, for which the mitochondrial sequences thought to be responsible have been described, and possible mechanisms by which nuclear restoration occurs in these systems can now be postulated.
Journal ArticleDOI
Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing
Zhonghua Wang,Yanjiao Zou,Xiaoyu Li,Qunyu Zhang,Letian Chen,Hao Wu,Dihua Su,Yuanling Chen,Jingxin Guo,Da Luo,Da Luo,Yunming Long,Yang Zhong,Yao-Guang Liu +13 more
TL;DR: It is shown in rice (Oryza sativa) with Boro II cytoplasm that an abnormal mitochondrial open reading frame, orf79, is cotranscribed with a duplicated atp6 (B-atp6) gene and encodes a cytotoxic peptide and plays an additional role in promoting the editing of atp 6 mRNAs, independent of its cleavage function.
Journal ArticleDOI
Male Sterility and Fertility Restoration in Crops
Letian Chen,Yao-Guang Liu +1 more
TL;DR: Recent research on CMS and EGMS systems in crops are reviewed, general models of male sterility and fertility restoration are summarized, and the evolutionary significance of these reproductive systems are discussed.
Journal ArticleDOI
A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants.
TL;DR: The isolation of a gene directly involved in the control of the expression of a cytoplasmic male sterility-encoding gene is reported, and the Petunia restorer of fertility gene product is a mitochondrially targeted protein that is almost entirely composed of 14 repeats of the 35-aa pentatricopeptide repeat motif.
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