Transport of the membrane glycoprotein of vesicular stomatitis virus to the cell surface in two stages by clathrin-coated vesicles.
TLDR
Pulse-chase experiments suggest that G is transported to the cell surface in two successive waves of clathrin-coated vesicles, with the early wave proposed to correspond to transport of G in coated vesicle from the endoplasmic reticulum to the Golgi apparatus, and the succeeding wave to transport from the Gol Gi apparatus to the plasma membrane.Abstract:
The G protein of vesicular stomatitis virus is a transmembrane glycoprotein that is transported from its site of synthesis in the rough endoplasmic reticulum to the plasma membrane via the Golgi apparatus. Pulse-chase experiments suggest that G is transported to the cell surface in two successive waves of clathrin-coated vesicles. The oligosaccharides of G protein carried in the early wave are of the "high-mannose" (G1) form, whereas the oligosaccharides in the second, later wave are of the mature "complex" (G2) form. the early wave is therefore proposed to correspond to transport of G in coated vesicles from the endoplasmic reticulum to the Golgi apparatus, and the succeeding wave to transport from the Golgi apparatus to the plasma membrane. The G1- and G2-containing coated vesicles appear to be structurally distinct, as judged by their differential precipitation by anticoated vesicle serum.read more
Citations
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The trans Golgi Network: Sorting at the Exit Site of the Golgi Complex
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Pre- and post-Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface.
Jaakko Saraste,Esa Kuismanen +1 more
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Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates
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O-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the Golgi apparatus.
D C Johnson,Patricia G. Spear +1 more
TL;DR: It is concluded that extension of O-linked oligosaccharide chains on HSV1 glycoproteins, and probably also attachment of the first O- linked sugars, occurs as a late posttranslational modification in the Golgi apparatus.
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Forty Years of Clathrin‐coated Vesicles
TL;DR: The purification of coated vesicles and the discovery of clathrin by Barbara Pearse in 1975 was a landmark in cell biology, but there are still a number of unanswered questions, including howClathrin‐mediated trafficking is regulated and how the machinery evolved.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction
John E. Heuser,Thomas S. Reese +1 more
TL;DR: During stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vESicles by way of intermediate cisternae.
Journal ArticleDOI
Coated pits, coated vesicles, and receptor-mediated endocytosis
TL;DR: It is now recognised that receptor-mediated endocytosis has a fundamental role in the growth, nutrition and differentiation of animal cells.
Journal ArticleDOI
Yolk protein uptake in the oocyte of the mosquito aedes aegypti. l.
Thomas F. Roth,Keith R. Porter +1 more
TL;DR: Preliminary radioautographs, and certain morphological features of the fat body, ovary, and midgut, suggest that the midGut is the principal site of yolk protein synthesis in the mosquito.
Journal ArticleDOI
Purification and Properties of an Endo-β-N-acetylglucosaminidase from Streptomyces griseus
Anthony L. Tarentino,Frank Maley +1 more
TL;DR: An enzyme that hydrolyzes di-N-acetylchitobiose linkages in oligosaccharides and glycoproteins was purified to homogeneity from cultural filtrates of Streptomyces griseus and appears to consist of a single polypeptide chain.