Patent
Ultra-high multiplex analytical systems and methods
Denis Zaccarin,Paul Lundquist,Peiqian Zhao,Frank Zhong,Stephen Turner,Yanqiao Huang,Pezhman Monadgemi,Ravi Saxena,Annette Grot,Aaron Rulison +9 more
TLDR
In this article, reflective optical components include reflective optical elements which redirect illumination light and light emitted from the discrete regions to more efficiently collect emitted light, which is used for single molecule reaction analysis.Abstract:
Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.read more
Citations
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Patent
Illumination of integrated analytical systems
TL;DR: In this paper, an analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding and a waveguide assembly for receiving optical illumination and introducing illumination into the device is described.
Patent
Integrated analytical system and method
TL;DR: An analytical assembly within a unified device structure for integration into an analytical system is described in this paper, where a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor.
Patent
Zero-mode waveguides with non-reflecting walls
TL;DR: In this article, the optical containment structures generally comprise zero-mode waveguide structures having non-reflective walls, which allow the preparation of optical containment regions in which the containment dimensions can be decoupled from the solution containment dimensions.
Patent
Compositions and methods for enrichment of nucleic acids
TL;DR: In this paper, the authors proposed methods for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acid sequence analysis, e.g., sequence segmentation.
Patent
Energy conversion cells using tapered waveguide spectral splitters
TL;DR: In this paper, an energy converter for converting multi-frequency radiant energy into electrical energy is disclosed, comprising a plurality of superposed lateral waveguides having photovoltaic energy transducers disposed within.
References
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Journal ArticleDOI
Real-Time DNA Sequencing from Single Polymerase Molecules
John Eid,Adrian Fehr,Jeremy Gray,Khai Luong,John Lyle,Geoff Otto,Paul Peluso,David R. Rank,Primo Baybayan,Brad Bettman,Arkadiusz Bibillo,Keith Bjornson,Bidhan Chaudhuri,Fred Christians,Ronald L. Cicero,Sonya Clark,Ravindra V. Dalal,Alex DeWinter,John Dixon,Mathieu Foquet,Alfred Gaertner,Paul Hardenbol,Cheryl Heiner,Kevin Hester,David P. Holden,Gregory J. Kearns,Xiangxu Kong,Ronald Kuse,Yves Lacroix,Steven Lin,Paul Lundquist,Congcong Ma,Patrick Marks,Mark Maxham,Devon Murphy,Insil Park,Thang Pham,Michael Phillips,Joy Roy,Robert Sebra,Gene Shen,Jon M. Sorenson,Austin B. Tomaney,Kevin Travers,Mark Trulson,John Vieceli,Jeffrey Wegener,Dawn Wu,Alicia Yang,Denis Zaccarin,Peter Zhao,Frank Zhong,Jonas Korlach,Stephen Turner +53 more
TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.
Journal ArticleDOI
Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations
Michael J. Levene,Jonas Korlach,Stephen Turner,Mathieu Foquet,Harold G. Craighead,Watt W. Webb +5 more
TL;DR: It is shown that arrays of zero-mode waveguides consisting of subwavelength holes in a metal film provide a simple and highly parallel means for studying single-molecule dynamics at micromolar concentrations with microsecond temporal resolution.
Journal ArticleDOI
Radiative decay engineering 3. Surface plasmon-coupled directional emission
TL;DR: A new method of fluorescence detection that promises to increase sensitivity by 20- to 1000-fold and decrease the contribution of sample autofluorescence to the detected signal is described, likely to find widespread applications throughout the biosciences.
Patent
Arrays of optical confinements and uses thereof
Stephen Turner,Jonas Korlach +1 more
TL;DR: In this paper, the present invention relates to optical confinements, methods of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions, and is particularly useful for high-throughput and low-cost single-molecular analysis.
Journal ArticleDOI
Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures
Jonas Korlach,Patrick Marks,Ronald L. Cicero,Jeremy Gray,Devon Murphy,Daniel B. Roitman,Thang Pham,Geoff Otto,Mathieu Foquet,Stephen Turner +9 more
TL;DR: Results presented here support the application of immobilized single DNA polymerases in ZMW arrays for long-read-length DNA sequencing.