Journal ArticleDOI
Using Mixed Self-Assembled Monolayers Presenting RGD and (EG)3OH Groups To Characterize Long-Term Attachment of Bovine Capillary Endothelial Cells to Surfaces
Carmichael Roberts,Christopher S. Chen,Milan Mrksich,Valerie Martichonok,Donald E. Ingber,George M. Whitesides +5 more
TLDR
Surfaces that promote the ligand-directed binding of cells and resist the cellular deposition of adhesive proteins are described, based on self-assembled monolayers of alkanethiolates on gold that present mixtures of arginine-glycine-aspartate, a tripeptide that promotes cell adhesion by binding to cell surface integrin receptors.Abstract:
This paper describes surfaces that promote the ligand-directed binding of cells and resist the cellular deposition of adhesive proteins. These surfaces are based on self-assembled monolayers (SAMs) of alkanethiolates on gold that present mixtures of arginine-glycine-aspartate (RGD), a tripeptide that promotes cell adhesion by binding to cell surface integrin receptors, and oligo(ethyleneglycol) moieties, groups that resist nonbiospecific adsorption of proteins and cells. Surface plasmon resonance (SPR) spectroscopy was used to measure the adsorption of carbonic anhydrase and fibrinogen to mixed SAMs comprising RGD groups ((EG)6OGRGD) and tri(ethylene glycol) groups ((EG)3OH); SAMs having values of the mole fraction of RGD (χRGD) ≤ 0.05 adsorbed nearly undetectable levels of carbonic anhydrase or fibrinogen. Bovine capillary endothelial cells attached and spread on SAMs at χRGD ≥ 0.00001, with spreading of cells reaching a maximum at χRGD ≥ 0.001. These mixed SAMs reduced the deposition of proteins by atta...read more
Citations
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Self-assembled monolayers of thiolates on metals as a form of nanotechnology.
Journal ArticleDOI
Soft Lithography in Biology and Biochemistry
TL;DR: Soft lithography offers the ability to control the molecular structure of surfaces and to pattern the complex molecules relevant to biology, to fabricate channel structures appropriate for microfluidics, and topattern and manipulate cells.
Journal ArticleDOI
RGD modified polymers: biomaterials for stimulated cell adhesion and beyond
TL;DR: The impacts of RGD peptide surface density, spatial arrangement as well as integrin affinity and selectivity on cell responses like adhesion and migration are discussed.
Journal ArticleDOI
Environmental sensing through focal adhesions
TL;DR: The mechanisms of such environmental sensing are discussed, based on the finely tuned crosstalk between the assembly of one type of integrin-based adhesion complex, namely focal adhesions, and the forces that are at work in the associated cytoskeletal network owing to actin polymerization and actomyosin contraction.
Journal ArticleDOI
Polymer Brushes via Surface-Initiated Controlled Radical Polymerization: Synthesis, Characterization, Properties, and Applications
Raphael Barbey,Laurent Lavanant,Dusko Paripovic,Nicolas Schüwer,Caroline Sugnaux,Stefano Tugulu,Harm-Anton Klok +6 more
TL;DR: This data indicates that self-Assembled Monolayers and Walled Carbon Nanotubes with high adhesion to Nitroxide-Mediated Polymerization have potential in the well-Defined Polymer Age.
References
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Integrins: versatility, modulation, and signaling in cell adhesion.
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New perspectives in cell adhesion : RGD and integrins
TL;DR: Together, the adhesion proteins and their receptors constitute a versatile recognition system providing cells with anchorage, traction for migration, and signals for polarity, position, differentiation, and possibly growth.
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Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule
TL;DR: The ability of fibronectin to bind cells can be accounted for by the tetrapeptide L-arginyl-glycyl- L-aspartyl-L-serine, a sequence which is part of the cell attachment domain of fibronsectin and present in at least five other proteins.
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Integrins and signal transduction pathways: the road taken
Edwin A. Clark,Joan S. Brugge +1 more
TL;DR: Recent advances in understanding of intracellular signal transduction pathways regulated by the integrin family of adhesion receptors are focused on.
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Self-assembled organic monolayers: model systems for studying adsorption of proteins at surfaces
TL;DR: Self-assembled monolayers of omega-functionalized long-chain alkanethiolates on gold films are excellent model systems with which to study the interactions of proteins with organic surfaces and the ability to create interfaces with similar structures and well-defined compositions should make it possible to test hypotheses concerning protein adsorption.