Vascular endothelial growth factor receptor localization and activation in human trophoblast and choriocarcinoma cells.
David Stephen Charnock-Jones,Andrew M. Sharkey,C. A. Boocock,Asif Ahmed,Robin Plevin,Napoleone Ferrara,Stephen K. Smith +6 more
TLDR
This study is the first demonstration of the presence of flt on non-endothelial cells in vivo and suggests a role for VEGF in the growth and differentiation of cytotrophoblast at implantation.Abstract:
Vascular endothelial growth factor (VEGF; also known as vascular permeability factor) is a secreted angiogenic growth factor. It is highly specific for endothelial cells, and its receptor, the fms-like tyrosine kinase (flt), has been localized only to endothelial cells in vivo. Here we describe the expression of mRNA encoding flt in human trophoblast as revealed by in situ hybridization. This mRNA is highly expressed in the cytotrophoblast shell and columns and also highly expressed by the extravillous trophoblast (EVT) in the maternal decidua both in the first trimester and at term. The trophoblast-like choriocarcinoma cell line BeWo also expresses this receptor and the related receptor, kinase domain-containing receptor (KDR), which is also a receptor for VEGF. Treatment of the cell line BeWo with VEGF165 stimulated 3H-thymidine incorporation and tyrosine phosphorylation of MAP (mitogen-activated protein) kinase in a time- and dose-dependent fashion. This study is the first demonstration of the presence of flt on non-endothelial cells in vivo and suggests a role for VEGF in the growth and differentiation of cytotrophoblast at implantation.read more
Citations
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Vascular endothelial growth factor (VEGF) and its receptors
TL;DR: Recent developments that have widened considerably the understanding of the mechanisms that control V EGF production and VEGF signal transduction are focused on and recent studies that have shed light on the mechanisms by which VEGf regulates angiogenesis are reviewed.
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Etiology and pathogenesis of preeclampsia: Current concepts
Gustaaf A. Dekker,Baha M. Sibai +1 more
TL;DR: The possibility of genetic imprinting should be considered in future genetic investigations of preeclampsia, as it is likely to reduce albumin’s antitoxic activity to a point at which very-low density lipoprotein toxicity is expressed.
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Vascular Endothelial Growth Factor Ligands and Receptors That Regulate Human Cytotrophoblast Survival Are Dysregulated in Severe Preeclampsia and Hemolysis, Elevated Liver Enzymes, and Low Platelets Syndrome
Yan Zhou,Michael T. McMaster,Kirstin Woo,Mary Janatpour,Jean Perry,Terhi Karpanen,Kari Alitalo,Caroline H. Damsky,Susan J. Fisher +8 more
TL;DR: VEGF family members regulate cytotrophoblast survival and that expression of a subset of family members is dysregulated in severe forms of preeclampsia, which is shown to be key regulators of conventional vasculogenesis and angiogenesis.
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VEGF receptor signal transduction.
TL;DR: Vascular endothelial growth factors (VEGFs) constitute an expanding family of growth factors that have received considerable attention from the basic research community but also from clinically active scientists and pharmaceutical companies.
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Comparative Developmental Anatomy of the Murine and Human Definitive Placentae
TL;DR: The developmental anatomy of the placenta between humans and mice is compared with emphasis on structures and cell types that might be analogous between the two species.
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