Journal ArticleDOI
Water‐soluble carbon nanotube‐enzyme conjugates as functional biocatalytic formulations
Prashanth Asuri,Sandeep S. Karajanagi,Edward Sellitto,Dae-Yun Kim,Ravi S. Kane,Jonathan S. Dordick +5 more
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TLDR
These water‐soluble carbon nanotube‐enzyme conjugates represent novel preparations that possess the virtues of both soluble and immobilized enzymes, thus providing a unique combination of useful attributes such as low mass transfer resistance, high activity and stability, and reusability.Abstract:
We report the activity, stability, and reusability of enzyme-carbon nanotube conjugates in aqueous solutions. A variety of enzymes were covalently attached to oxidized multi-walled carbon nanotubes (MWNTs). These conjugates were soluble in aqueous buffer, retained a high fraction of their native activity, and were stable at higher temperatures relative to their solution phase counterparts. Furthermore, the high surface area of MWNTs afforded high enzyme loadings, yet the intrinsic high length of the MWNT led to facile filtration. These water-soluble carbon nanotube-enzyme conjugates represent novel preparations that possess the virtues of both soluble and immobilized enzymes, thus providing a unique combination of useful attributes such as low mass transfer resistance, high activity and stability, and reusability.read more
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An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
Nur Royhaila Mohamad,Nur Haziqah Che Marzuki,Nor Aziah Buang,Fahrul Huyop,Roswanira Abdul Wahab +4 more
TL;DR: Characterization technologies at the nanoscale level to study enzymes immobilized on surfaces are crucial to obtain valuable qualitative and quantitative information, including morphological visualization of the immobilized enzymes, to assess efficacy of an immobilization technique and development of future enzyme immobilization strategies.
Journal ArticleDOI
Enzyme–MOF (metal–organic framework) composites
Xizhen Lian,Yu Fang,Elizabeth A. Joseph,Qi Wang,Jialuo Li,Sayan Banerjee,Christina T. Lollar,Xuan Wang,Hong-Cai Zhou +8 more
TL;DR: This review summarizes recent developments of MOF-enzyme composites with special emphasis on preparative techniques and the synergistic effects of enzymes and MOFs.
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Fast-growing field of magnetically recyclable nanocatalysts.
Dong Wang,Didier Astruc +1 more
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Emerging Applications of Carbon Nanotubes
Jan M. Schnorr,Timothy M. Swager +1 more
TL;DR: In this article, a review highlights illustrative examples from these areas to give an overview of applications of carbon nanotubes (CNTs) in various areas of computer vision, catalysis and sensing, filters and mechanical and biomedical applications.
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Carbon Nanotubes: A Review on Structure and Their Interaction with Proteins
TL;DR: Advances in methodology for enzyme immobilization on carbon nanotubes are focused on, which shows the high surface-to-volume ratio offered by nanoparticles resulted in the concentration of the immobilized entity being considerably higher than that afforded by other materials.
References
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