Yeast mutants auxotrophic for choline or ethanolamine.
TLDR
Three mutants of the yeast Saccharomyces cerevisiae which require exogenous ethanolamine or choline were isolated and the lipid composition suggests that cho1 mutants do not synthesize phosphatidylserine under any growth conditions.Abstract:
Three mutants of the yeast Saccharomyces cerevisiae which require exogenous ethanolamine or choline were isolated. The mutants map to a single locus (cho1) on chromosome V. The lipid composition suggests that cho1 mutants do not synthesize phosphatidylserine under any growth conditions. If phosphatidylethanolamine or phosphatidylcholine, which are usually derived from phosphatidylserine, were synthesized from exogenous ethanolamine or choline, the mutants grew and divided relatively normally. However, mitochondrial abnormalities were evident even when ethanolamine and choline were supplied. Diploids homozygous for the cho1 mutation were defective in sporulation. Growth on nonfermentable carbon sources was slow, and a high proportion of respiratory-deficient (petite) cells were generated in cho1 cultures.read more
Citations
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Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.
TL;DR: Three new dominant drug resistance cassettes have unique antibiotic resistance phenotypes and do not affect growth when inserted into the ho locus, which make the cassettes ideally suited for creating S. cerevisiae strains with multiple mutations within a single strain.
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Biochemistry, Cell Biology and Molecular Biology of Lipids of Saccharomyces cerevisiae
TL;DR: Current knowledge about lipid biosynthetic pathways in S. cerevisiae is summarized and characteristic features of the gene products involved are described, focusing on recent discoveries in these fields.
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The Saccharomyces cerevisiae Lipin Homolog Is a Mg2+-dependent Phosphatidate Phosphatase Enzyme
TL;DR: Lin et al. as mentioned in this paper identified the Saccharomyces cerevisiae PAH1 (previously known as SMP2) gene that encodes Mg2+dependent PA phosphatase using amino acid sequence information derived from a purified preparation of the enzyme.
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Metabolism and Regulation of Glycerolipids in the Yeast Saccharomyces cerevisiae
TL;DR: Genome-wide genetic analysis indicates coordinate regulation between glycerolipid metabolism and a broad spectrum of metabolic pathways.
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Genetic map of Saccharomyces cerevisiae.
R K Mortimer,D Schild +1 more
TL;DR: Although all of the data presented in this article are from tetrad analyses, many other techniques have been used to assign genes to chromosomes or to specific chromosome arms, including aneuploid analysis, mitotic recombination analysis,Mitotic chromosome loss or nondisjunction, and random spore analysis.
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