Alexis Vallée-Bélisle

TL;DR: A novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid sequences, proteins, small molecules, and inorganic ions is demonstrated.

TL;DR: Programmable DNA-based nanoswitches whose closing/opening can be triggered over specific different pH windows are designed and it is demonstrated that by simply changing the relative content of TAT/CGC triplets in the switches, they can rationally tune their pH dependence over more than 5 pH units.

TL;DR: The goal of the efforts is to set uniform standards for the experimental community and to initiate an accumulating, self‐consistent data set that will aid ongoing efforts to understand the folding process.

TL;DR: The development of advanced optical readout mechanisms, including genetically encoded fluorophores, and electrochemical readouts supporting detection directly in highly complex sample matrices, switch-based sensors have already seen deployment in applications ranging from real time, in vivo imaging to the continuous monitoring of drugs in blood serum.

TL;DR: The generality of the PCA strategy is demonstrated with examples of assays that are designed on the basis of other enzymes including glycinamide ribonucleotide transformylase, aminoglycoside kinase, and hygromycin B kinase.