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Alfred Pingoud
Researcher at University of Giessen
Publications - 159
Citations - 8472
Alfred Pingoud is an academic researcher from University of Giessen. The author has contributed to research in topics: Restriction enzyme & DNA. The author has an hindex of 48, co-authored 159 publications receiving 8134 citations. Previous affiliations of Alfred Pingoud include Pierre-and-Marie-Curie University.
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Journal ArticleDOI
Real‐Time Polymerase Chain Reaction
Jochen Wilhelm,Alfred Pingoud +1 more
TL;DR: A survey of the instruments and assay formats available and the pros and cons of each is provided and examples of applications of this extremely versatile technique are given that demonstrate the enormous impact of real‐time PCR on life sciences and molecular medicine.
Journal ArticleDOI
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
Richard J. Roberts,Marlene Belfort,Timothy H. Bestor,Ashok S. Bhagwat,Thomas A. Bickle,Jurate Bitinaite,Robert Blumenthal,Sergey Kh. Degtyarev,David T. F. Dryden,Kevin Dybvig,Keith Firman,Elizaveta S. Gromova,Richard I. Gumport,Stephen E. Halford,Stanley Hattman,Joseph Heitman,David P. Hornby,Arvydas Janulaitis,Albert Jeltsch,Jytte Josephsen,Antal Kiss,Todd R. Klaenhammer,Ichizo Kobayashi,Huimin Kong,Detlev H. Krüger,Sanford A. Lacks,Martin G. Marinus,Michiko Miyahara,Richard D. Morgan,Noreen E. Murray,Valakunja Nagaraja,Andrzej Piekarowicz,Alfred Pingoud,Elisabeth A. Raleigh,Desirazu N. Rao,Norbert O. Reich,Repin Vladimir E,Eric U. Selker,Pang-Chui Shaw,Daniel C. Stein,Barry L. Stoddard,Waclaw Szybalski,Thomas A. Trautner,James L. Van Etten,Jorge M. B. Vítor,Geoffrey G. Wilson,Shuang Yong Xu +46 more
TL;DR: In this article, a nomenclature for restriction endonucleases, DNA methyltransferases, homing endon nucleases and related genes and gene products is described.
Journal ArticleDOI
Structure and function of type II restriction endonucleases
Alfred Pingoud,Albert Jeltsch +1 more
TL;DR: Type II restriction endonucleases recognize short, usually palindromic, sequences of 4-8 bp and, in the presence of Mg(2+), cleave the DNA within or in close proximity to the recognition sequence.
Journal ArticleDOI
Type II restriction endonucleases: structure and mechanism.
TL;DR: A major emphasis of this review will be on target site location and the mechanism of catalysis, two problems currently being addressed in the literature.
Journal ArticleDOI
Recognition and cleavage of DNA by type-II restriction endonucleases.
Alfred Pingoud,Albert Jeltsch +1 more
TL;DR: Structural similarities identified between EcoRI, EcoRV, BamHI, PvuII and Cfr10I suggest that many type II restriction endonucleases are not only functionally but also evolutionarily related.