Shuang Yong Xu

TL;DR: In this article, a nomenclature for restriction endonucleases, DNA methyltransferases, homing endon nucleases and related genes and gene products is described.

TL;DR: The structure of a Type III R–M system, consisting of the entire EcoP15I complex (Mod2Res1) bound to DNA, is reported, which suggests how ATP hydrolysis is coupled to long-range diffusion of a helicase on DNA, and how a dimeric methyltransferase functions to methylate only one of the two DNA strands.

TL;DR: The cloning and expression of the CviPII DNA nicking and modification system encoded by chlorella virus NYs-1 is described and can be used to generate single-stranded DNAs for isothermal strand-displacement amplification.

TL;DR: The identification of a large pool of previously unknown natural NEases and engineered NEases provides more ‘tools’ for DNA manipulation and molecular diagnostics and the small modular HNH nicking domain can be used to generate rare NEases applicable to targeted genome editing.

TL;DR: The crystal structure of a putative HNH endonuclease, Gmet_0936 protein from Geobacter metallireducens GS-15, has been determined at 2.6 Å resolution using single-wavelength anomalous dispersion method, and shows a weak DNA binding activity in a DNA mobility shift assay and a weak Mn2+-dependent nicking activity on supercoiled plasmids in low pH buffers.