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Alphonsus H. C. Ng
Researcher at Institute for Systems Biology
Publications - 36
Citations - 2243
Alphonsus H. C. Ng is an academic researcher from Institute for Systems Biology. The author has contributed to research in topics: Digital microfluidics & T cell. The author has an hindex of 21, co-authored 32 publications receiving 1701 citations. Previous affiliations of Alphonsus H. C. Ng include University of Toronto & California Institute of Technology.
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Journal ArticleDOI
Immunoassays in microfluidic systems
TL;DR: This review, which summarizes developments in microfluidics-based immunoassays since 2000, includes four sections, focusing on the configurations of immuno ASSays that have been implemented in micro fluidics, the main fluid handling modalities that have be used for microfluidity immunoASSays, multiplexed immunoAssays in micro fluididic platforms, and the emergence of label-free detection techniques.
Journal ArticleDOI
Key Parameters of Tumor Epitope Immunogenicity Revealed Through a Consortium Approach Improve Neoantigen Prediction
Daniel K. Wells,Marit M. van Buuren,Kristen K. Dang,Vanessa M. Hubbard-Lucey,Kathleen C. F. Sheehan,Katie M. Campbell,Andrew Lamb,Jeffrey P. Ward,John Sidney,Ana Belen Blazquez,Andrew J. Rech,Jesse M. Zaretsky,Begonya Comin-Anduix,Alphonsus H. C. Ng,William Chour,Thomas Yu,Hira Rizvi,Jia M. Chen,Patrice Manning,Gabriela Steiner,Xengie Doan,Aly A. Khan,Amit A. Lugade,Ana M. Mijalkovic Lazic,Angela Frentzen,Arbel D. Tadmor,Ariella Sasson,Arjun A. Rao,Baikang Pei,Barbara Schrörs,Beata Berent-Maoz,Beatriz M. Carreno,Bin Song,Bjoern Peters,Bo Li,Brandon W. Higgs,Brian Stevenson,Christian Iseli,Christopher A. Miller,Christopher A. Morehouse,Cornelis J. M. Melief,Cristina Puig-Saus,Daphne van Beek,David Balli,David Gfeller,David Haussler,Dirk Jäger,Eduardo Cortes,Ekaterina Esaulova,Elham Sherafat,Francisco Arcila,Gabor Bartha,Geng Liu,George Coukos,Guilhem Richard,Han Chang,Han Si,Inka Zörnig,Ioannis Xenarios,Ion I. Mandoiu,Irsan Kooi,James P. Conway,Jan H. Kessler,Jason A. Greenbaum,Jason Perera,Jason Harris,Jasreet Hundal,Jennifer M. Shelton,Jianmin Wang,Jiaqian Wang,Joel Greshock,Jonathon Blake,Joseph D. Szustakowski,Julia Kodysh,Juliet Forman,Lei Wei,Leo J. Lee,Lorenzo F. Fanchi,Maarten Slagter,Maren Lang,Markus Mueller,Martin Löwer,Mathias Vormehr,Maxim N. Artyomov,Michael A. Kuziora,Michael F. Princiotta,Michal Bassani-Sternberg,Mignonette H. Macabali,Milica R. Kojicic,Naibo Yang,Nevena M. Ilic Raicevic,Nicolas Guex,Nicolas Robine,Niels Halama,Nikola M. Skundric,Ognjen Milicevic,Pascal Gellert,Patrick Jongeneel,Pornpimol Charoentong,Pramod K. Srivastava,Prateek Tanden,Priyanka Shah,Qiang Hu,Ravi Gupta,Richard Chen,Robert A. Petit,Robert Ziman,Rolf Hilker,Sachet A. Shukla,Sahar Al Seesi,Sean Michael Boyle,Si Qiu,Siranush Sarkizova,Sofie R. Salama,Song Liu,Song Wu,Sriram Sridhar,Steven L. C. Ketelaars,Suchit Jhunjhunwala,Tatiana Shcheglova,Thierry Schuepbach,Todd Creasy,Veliborka Josipovic,Vladimir B. Kovacevic,Weixuan Fu,Willem-Jan Krebber,Yi-Hsiang Hsu,Yinong Sebastian,Zeynep Kosaloglu Yalcin,Zhiqin Huang,Taha Merghoub,Taha Merghoub,Justin Guinney,Justin Guinney,Adam Kolom,Cheryl Selinsky,Antoni Ribas,Antoni Ribas,Matthew D. Hellmann,Nir Hacohen,Nir Hacohen,Alessandro Sette,Alessandro Sette,James R. Heath,Nina Bhardwaj,Fred Ramsdell,Robert D. Schreiber,Ton N. Schumacher,Pia Kvistborg,Nadine Defranoux +149 more
TL;DR: A model of tumor epitope immunogenicity was developed that filtered out 98% of non-immunogenic peptides with a precision above 0.70 and was validated in an independent cohort of 310 epitopes prioritized from tumor sequencing data and assessed for T cell binding.
Journal ArticleDOI
Digital Microfluidic Magnetic Separation for Particle-Based Immunoassays
TL;DR: The first particle-based immunoassay on DMF without the aid of oil carrier fluid to enable droplet movement is reported, allowing the realization of a novel on-chip particle separation and resuspension method capable of removing greater than 90% of unbound reagents in one step.
Journal ArticleDOI
Automated digital microfluidic platform for magnetic-particle-based immunoassays with optimization by design of experiments.
Kihwan Choi,Alphonsus H. C. Ng,Ryan Fobel,David Chang-Yen,Lyle Yarnell,Elroy L. Pearson,Carl M. Oleksak,Andrew T. Fischer,Robert P. Luoma,John M. Robinson,Julie Audet,Aaron R. Wheeler +11 more
TL;DR: It is proposed that this new platform paves the way for a benchtop tool that is useful for implementing immunoassays in near-patient settings, including community hospitals, physicians' offices, and small clinical laboratories.
Journal ArticleDOI
A digital microfluidic system for serological immunoassays in remote settings
Alphonsus H. C. Ng,Ryan Fobel,Christian Fobel,Julian Lamanna,Darius G. Rackus,Aimee Summers,Christopher Dixon,Michael D. M. Dryden,Charis Lam,Man Ho,Nooman S. Mufti,Victor Lee,Mohd Afiq Mohd Asri,Edward A. Sykes,M. Dean Chamberlain,Rachael Joseph,Maurice Ope,Heather M. Scobie,Alaine Knipes,Paul A. Rota,Nina Marano,Paul M. Chege,Mary Njuguna,Rosemary Nzunza,Ngina Kisangau,John Kiogora,Michael Karuingi,John Wagacha Burton,Peter Borus,Eugene Lam,Aaron R. Wheeler +30 more
TL;DR: A compact and portable, field-deployable, point-of-care system relying on digital microfluidics that can rapidly test a small volume of capillary blood for disease-specific antibodies for measles and rubella is described.