James D. White

TL;DR: The draft sequence of the M. truncatula genome sequence is described, a close relative of alfalfa (Medicago sativa), a widely cultivated crop with limited genomics tools and complex autotetraploid genetics, which provides significant opportunities to expand al falfa’s genomic toolbox.

TL;DR: Using a strategy to isolate double‐stranded RNA from individual plants and convert this to cDNA with a unique four nucleotide Tag at each end, this technology will be extremely useful in understanding the full scope of plant virus biodiversity.

TL;DR: A sequencing strategy that combines these isolation and sequencing methods with initial shotgun cloning followed by a short oligonucleotide primer walking approach for both contig joining and proofreading is described, which significantly increase the rate and ease of DNA sequence data acquisition, as well as improve the extent and accuracy of the sequence data.

TL;DR: The methods provided by the manufacturer for genome sequencing using the 454/Roche GS‐20 and GS‐FLX instruments are robust, but in an effort to streamline them for automation, several novel changes are incorporated and several extraneous steps are deleted.

TL;DR: FELINES was shown to be useful for characterizing branchsites, polypyrimidine tracts and 5' and 3' splice sites in the intron databases and exonic splicing enhancers in S.pombe exons.