K
Ken Fujii
Researcher at Institute of Medical Science
Publications - 24
Citations - 3507
Ken Fujii is an academic researcher from Institute of Medical Science. The author has contributed to research in topics: Influenza A virus & Orthomyxoviridae. The author has an hindex of 21, co-authored 23 publications receiving 3249 citations. Previous affiliations of Ken Fujii include University of Tokyo.
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Journal ArticleDOI
In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses
Yasushi Itoh,Kyoko Shinya,Maki Kiso,Tokiko Watanabe,Yoshihiro Sakoda,Masato Hatta,Yukiko Muramoto,Daisuke Tamura,Yuko Sakai-Tagawa,Takeshi Noda,Saori Sakabe,Masaki Imai,Yasuko Hatta,Shinji Watanabe,Chengjun Li,Shinya Yamada,Ken Fujii,Shin Murakami,Hirotaka Imai,Satoshi Kakugawa,Mutsumi Ito,Ryo Takano,Kiyoko Iwatsuki-Horimoto,Masayuki Shimojima,Taisuke Horimoto,Hideo Goto,Kei Takahashi,Akiko Makino,Hirohito Ishigaki,Misako Nakayama,Masatoshi Okamatsu,Kazuo Takahashi,David Warshauer,Peter A. Shult,Reiko Saito,Hiroshi Suzuki,Yousuke Furuta,Makoto Yamashita,Keiko Mitamura,Kunio Nakano,Morio Nakamura,Rebecca A. Brockman-Schneider,Hiroshi Mitamura,Masahiko Yamazaki,Norio Sugaya,M. Suresh,Makoto Ozawa,Makoto Ozawa,Gabriele Neumann,James E. Gern,Hiroshi Kida,Kazumasa Ogasawara,Yoshihiro Kawaoka +52 more
TL;DR: It is shown that CA04 is sensitive to approved and experimental antiviral drugs, suggesting that these compounds could function as a first line of defence against the recently declared S-OIV pandemic.
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Influenza Virus-Host Interactome Screen as a Platform for Antiviral Drug Development
Tokiko Watanabe,Eiryo Kawakami,Jason E. Shoemaker,Tiago J. S. Lopes,Yukiko Matsuoka,Yuriko Tomita,Hiroko Kozuka-Hata,Takeo Gorai,Tomoko Kuwahara,Eiji Takeda,Atsushi Nagata,Ryo Takano,Maki Kiso,Makoto Yamashita,Yuko Sakai-Tagawa,Hiroaki Katsura,Naoki Nonaka,Hiroko Fujii,Ken Fujii,Yukihiko Sugita,Takeshi Noda,Hideo Goto,Satoshi Fukuyama,Shinji Watanabe,Gabriele Neumann,Masaaki Oyama,Hiroaki Kitano,Yoshihiro Kawaoka +27 more
TL;DR: A network of virus-host protein interactions based on the stage of the viral life cycle affected upon host factor downregulation is generated and several proteins, notably Golgi-specific brefeldin A-resistant guanine nucleotide exchange factor 1 and JAK1, are validated as potential antiviral drug targets.
Journal ArticleDOI
Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA
Eiryo Kawakami,Tokiko Watanabe,Ken Fujii,Hideo Goto,Shinji Watanabe,Takeshi Noda,Yoshihiro Kawaoka +6 more
TL;DR: A novel strand-specific real-time RT-PCR method was established to quantify the three types of influenza viral RNA (vRNA, cRNA, and mRNA) separately, based on reverse transcription using tagged primers to add a 'tag' sequence at the 5' end and the hot-start method.
Journal ArticleDOI
Contributions of Two Nuclear Localization Signals of Influenza A Virus Nucleoprotein to Viral Replication
Makoto Ozawa,Ken Fujii,Yukiko Muramoto,Shinya Yamada,Seiya Yamayoshi,Ayato Takada,Hideo Goto,Taisuke Horimoto,Yoshihiro Kawaoka +8 more
TL;DR: The results indicate that while the unconventional NLS is not essential for viral replication, it is necessary for efficient viral mRNA synthesis and was essential for vRNA transcription and NP's nucleolar accumulation.
Journal ArticleDOI
Hierarchy among viral RNA (vRNA) segments in their role in vRNA incorporation into influenza A virions.
Yukiko Muramoto,Ayato Takada,Ken Fujii,Takeshi Noda,Kiyoko Iwatsuki-Horimoto,Shinji Watanabe,Taisuke Horimoto,Hiroshi Kida,Yoshihiro Kawaoka +8 more
TL;DR: A hierarchy among vRNA segments for virion incorporation is suggested and may imply intersegment association of vRNAs during virus assembly.