Other affiliations: Kurukshetra University, Central Drug Research Institute, University of Louisville ...read more
Bio: Manish Kumar is an academic researcher from University of Petroleum and Energy Studies. The author has contributed to research in topics: Medicine & Computer science. The author has an hindex of 61, co-authored 1425 publications receiving 21762 citations. Previous affiliations of Manish Kumar include Kurukshetra University & Central Drug Research Institute.
Papers published on a yearly basis
Johns Hopkins University1, Johns Hopkins University School of Medicine2, University of Toronto3, University of La Frontera4, Imperial College London5, Post Graduate Institute of Medical Education and Research6, Armed Forces Medical College7, National Institute of Mental Health and Neurosciences8, Hong Kong University of Science and Technology9, University of Maryland, Baltimore10
TL;DR: A draft map of the human proteome is presented using high-resolution Fourier-transform mass spectrometry to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-c coding RNAs and upstream open reading frames.
Abstract: The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease.
TL;DR: An overview of the status and recent applications of mass spectrometry for microbial identification is provided and the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi is explored.
Abstract: Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor and costs involved. The technology has been readily imbibed by microbiologists who have reported usage of MALDI-TOF MS for a number of purposes like, microbial identification and strain typing, epidemiological studies, detection of biological warfare agents, detection of water- and food-borne pathogens, detection of antibiotic resistance and detection of blood and urinary tract pathogens etc. The limitation of the technology is that identification of new isolates is possible only if the spectral database contains peptide mass fingerprints of the type strains of specific genera/species/subspecies/strains. This review provides an overview of the status and recent applications of mass spectrometry for microbial identification. It also explores the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi.
TL;DR: In this article, the authors highlighted the importance of digital change detection techniques for nature and location of change of the Hawalbagh block in district Almora, Uttarakhand, India.
Abstract: Digital change detection techniques by using multi-temporal satellite imagery helps in understanding landscape dynamics. The present study illustrates the spatio-temporal dynamics of land use/cover of Hawalbagh block of district Almora, Uttarakhand, India. Landsat satellite imageries of two different time periods, i.e., Landsat Thematic Mapper (TM) of 1990 and 2010 were acquired by Global Land Cover Facility Site (GLCF) and earth explorer site and quantify the changes in the Hawalbagh block from 1990 to 2010 over a period of 20 years. Supervised classification methodology has been employed using maximum likelihood technique in ERDAS 9.3 Software. The images of the study area were categorized into five different classes namely vegetation, agriculture, barren, built-up and water body. The results indicate that during the last two decades, vegetation and built-up land have been increased by 3.51% (9.39 km2) and 3.55% (9.48 km2) while agriculture, barren land and water body have decreased by 1.52% (4.06 km2), 5.46% (14.59 km2) and 0.08% (0.22 km2), respectively. The paper highlights the importance of digital change detection techniques for nature and location of change of the Hawalbagh block.
TL;DR: In this article, the authors reviewed existing research that provides reliable and quantitative information on pharmaceuticals, personal care products (PCPs), endocrine disrupting compounds (EDCs), pharmaceuticals (PhACs) and their transformation products, whose occurrence at trace levels in treated wastewater is of concern for human health and the aquatic ecosystem.
Abstract: Emerging contaminants (ECs), such as personal care products (PCPs), endocrine disrupting compounds (EDCs), pharmaceuticals (PhACs) and their transformation products,whose occurrence at trace levels in treated wastewater is of concern for human health and the aquatic ecosystem. Due to the relatively new introduction or detection of these pollutants, there exists a gap in the knowledge on their fate, behaviors and effects, as well as on treatment technologies for their efficient removal. Furthermore, despite the advances in treatment technologies, the design of existing treatment plants are not suited to remove these ECs, in addition to there being a lack of published health standards that provide guideline in treating these pollutants. Many new ECs are being introduced into the environment without detection. In these context, this paper reviews existing research that provide reliable and quantitative information on pharmaceuticals, PCPs and EDCs and their concentrations in surface water, ground water, drinking water and treated wastewater and the removal efficiency of different treatment processes for different emerging pollutants, with a focus on recent studies regarding the fate and behavior of the contaminants in wastewater treatment plants and in the environment as well. The paper also highlights various biological and chemical treatment techniques and their drawbacks. Also, this review discusses the scope of future research on ECs.
TL;DR: A study of the hydrogeochemical processes in the national capital territory of India was carried out with the objective of identifying the geochemical processes and their relation with groundwater quality as well as to get an insight into the hydrochemical evalution of groundwater as mentioned in this paper.
Abstract: A study of the hydrogeochemical processes in the national capital territory of India was carried out with the objective of identifying the geochemical processes and their relation with groundwater quality as well as to get an insight into the hydrochemical evalution of groundwater. Salinity and nitrate contamination are the two major problems in the area, which is alarming considering the use of this water for drinking. Various graphical plots and statistical analyses have been carried out using chemical data to deduce a hydrochemical evaluation of the aquifer system based on the ionic constituents, water types, hydrochemical facies and factors controlling groundwater quality. The prevailing hydrochemical processes operating here are simple dissolution, mixing, weathering of carbonate minerals, locally known as ‘kankar’, and of silicate, ion exchange, and surface water interaction. Limited reverse ion exchange has been noticed in a few parts of the study area especially in post-monsoon periods. Periodic seasonal switch-over has been clearly noticed in these hydrogeochemical processes that control groundwater quality of the area. The number of factors that control the overall mineralization and water quality of Delhi vary from season to season. In pre-monsoon, there are four factors while in post-monsoon it increases to five. The study highlights the descriptive capabilities of conventional and multivariate techniques as effective tools in groundwater evaluation.
TL;DR: There is, I think, something ethereal about i —the square root of minus one, which seems an odd beast at that time—an intruder hovering on the edge of reality.
Abstract: There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality. Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …
28 Jul 2005
TL;DR: In this paper, a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level.
Abstract: Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.