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Nozomu Yachie
Researcher at University of Tokyo
Publications - 74
Citations - 3578
Nozomu Yachie is an academic researcher from University of Tokyo. The author has contributed to research in topics: Gene & CRISPR. The author has an hindex of 21, co-authored 62 publications receiving 2672 citations. Previous affiliations of Nozomu Yachie include Keio University & University of British Columbia.
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Journal ArticleDOI
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
Keiji Nishida,Takayuki Arazoe,Nozomu Yachie,Nozomu Yachie,Nozomu Yachie,Satomi Banno,Mika Kakimoto,Mayura Tabata,Masao Mochizuki,Aya Miyabe,Michihiro Araki,Kiyotaka Y. Hara,Zenpei Shimatani,Akihiko Kondo +13 more
TL;DR: The toxicity associated with the nuclease-based CRISPR/Cas9 system was greatly reduced in the Target-AID complexes, and it was demonstrated that off-target effects were comparable to those of conventional CRISpr/Cas systems, with a reduced risk of indel formation.
Journal ArticleDOI
Engineered CRISPR-Cas9 nuclease with expanded targeting space
Hiroshi Nishimasu,Xi Shi,Xi Shi,Soh Ishiguro,Soh Ishiguro,Linyi Gao,Linyi Gao,Seiichi Hirano,Sae Okazaki,Taichi Noda,Omar O. Abudayyeh,Omar O. Abudayyeh,Omar O. Abudayyeh,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Hideto Mori,Hideto Mori,Seiya Oura,Benjamin Ray Holmes,Benjamin Ray Holmes,Mamoru Tanaka,Motoaki Seki,Hisato Hirano,Hiroyuki Aburatani,Ryuichiro Ishitani,Masahito Ikawa,Masahito Ikawa,Nozomu Yachie,Feng Zhang,Osamu Nureki +30 more
TL;DR: A rationally engineered SpCas9 variant (SpCas9-NG) that can recognize relaxed NG PAMs is reported, which is a powerful addition to the CRISPR-Cas9 genome engineering toolbox and will be useful in a broad range of applications, from basic research to clinical therapeutics.
Journal ArticleDOI
Widespread Macromolecular Interaction Perturbations in Human Genetic Disorders
Nidhi Sahni,Song Yi,Mikko Taipale,Juan I. Fuxman Bass,Jasmin Coulombe-Huntington,Fan Yang,Fan Yang,Jian Peng,Jochen Weile,Jochen Weile,Georgios I. Karras,Yang Wang,István Kovács,István Kovács,Atanas Kamburov,Irina Krykbaeva,Mandy H. Y. Lam,George Tucker,Vikram Khurana,Amitabh Sharma,Amitabh Sharma,Yang-Yu Liu,Yang-Yu Liu,Nozomu Yachie,Nozomu Yachie,Quan Zhong,Yun Shen,Alexandre Palagi,Adriana San-Miguel,Changyu Fan,Dawit Balcha,Amélie Dricot,Daniel M. Jordan,Jennifer M. Walsh,Akash A. Shah,Xinping Yang,Ani K. Stoyanova,Alex Leighton,Michael A. Calderwood,Yves Jacob,Yves Jacob,Michael E. Cusick,Kourosh Salehi-Ashtiani,Luke Whitesell,Shamil R. Sunyaev,Shamil R. Sunyaev,Bonnie Berger,Albert-László Barabási,Albert-László Barabási,Benoit Charloteaux,David E. Hill,Tong Hao,Frederick P. Roth,Frederick P. Roth,Frederick P. Roth,Yu Xia,Yu Xia,Albertha J.M. Walhout,Albertha J.M. Walhout,Susan Lindquist,Susan Lindquist,Marc Vidal +61 more
TL;DR: This work functionally profile several thousand missense mutations across a spectrum of Mendelian disorders using various interaction assays, suggesting that disease-associated alleles that perturb distinct protein activities rather than grossly affecting folding and stability are relatively widespread.
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Base editors for simultaneous introduction of C-to-T and A-to-G mutations
Rina C. Sakata,Rina C. Sakata,Soh Ishiguro,Soh Ishiguro,Hideto Mori,Hideto Mori,Mamoru Tanaka,Kenji Tatsuno,Hiroki Ueda,Shogo Yamamoto,Motoaki Seki,Nanami Masuyama,Nanami Masuyama,Keiji Nishida,Hiroshi Nishimasu,Kazuharu Arakawa,Akihiko Kondo,Osamu Nureki,Masaru Tomita,Hiroyuki Aburatani,Nozomu Yachie +20 more
TL;DR: A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminationase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A- to-G editing activity at 47 genomic targets.
Journal ArticleDOI
Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae.
Akiko Soma,Akinori Onodera,Junichi Sugahara,Akio Kanai,Nozomu Yachie,Masaru Tomita,Fujio Kawamura,Yasuhiko Sekine +7 more
TL;DR: Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.