Fan Yang

TL;DR: This work functionally profile several thousand missense mutations across a spectrum of Mendelian disorders using various interaction assays, suggesting that disease-associated alleles that perturb distinct protein activities rather than grossly affecting folding and stability are relatively widespread.

TL;DR: This work cloned full-length open reading frames of alternatively spliced transcripts for a large number of human genes and used protein-protein interaction profiling to functionally compare hundreds of protein isoform pairs, revealing a widespread expansion of protein interaction capabilities through alternative splicing and suggesting that many alternative "isoforms" are functionally divergent (i.e., "functional alloforms").

TL;DR: It is found that NLRP3 deficiency ameliorated cerebral injury in mice after ischemic stroke by reducing infarcts and blood–brain barrier (BBB) damage and that NOX2 deficiency improved outcomes after ischemia stroke by mediatingNLRP3 signaling.

TL;DR: It is shown that patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) who develop coronav virus disease 2019 (COVID-19) display early recruitment of B cells expressing a limited subset of IGHV genes, progressing to a highly polyclonal response of B Cells with broader I GHV gene usage and extensive class switching to IgG and IgA subclasses with limited somatic hypermutation in the initial weeks of infection.

TL;DR: A deep mutational scanning framework is developed that produces exhaustive maps for human missense variants by combining random codon mutagenesis and multiplexed functional variation assays with computational imputation and refinement.