P
Pradip Gyawali
Researcher at University of Queensland
Publications - 50
Citations - 2079
Pradip Gyawali is an academic researcher from University of Queensland. The author has contributed to research in topics: Wastewater & Indicator bacteria. The author has an hindex of 19, co-authored 48 publications receiving 1164 citations. Previous affiliations of Pradip Gyawali include Commonwealth Scientific and Industrial Research Organisation.
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Journal ArticleDOI
Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater.
Warish Ahmed,Paul M. Bertsch,Aaron Bivins,Kyle Bibby,Kata Farkas,Amy Gathercole,Eiji Haramoto,Pradip Gyawali,Asja Korajkic,Brian R. McMinn,Jochen F. Mueller,Stuart L. Simpson,Wendy Smith,Erin M. Symonds,Kevin V. Thomas,Rory Verhagen,Masaaki Kitajima +16 more
TL;DR: The results suggest that absorption-extraction methods with minimal pretreatment or without manipulation can provide suitably rapid, cost-effective and relatively straightforward recovery of enveloped viruses in wastewater.
Journal ArticleDOI
Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology.
Warish Ahmed,Paul M. Bertsch,Kyle Bibby,Eiji Haramoto,Joanne Hewitt,Flavia Huygens,Pradip Gyawali,Asja Korajkic,Shane Riddell,Samendra P. Sherchan,Stuart L. Simpson,Kwanrawee Sirikanchana,Erin M. Symonds,Rory Verhagen,Seshadri S. Vasan,Masaaki Kitajima,Aaron Bivins +16 more
TL;DR: Wastewater-based epidemiology (WBE) demonstrates potential for COVID-19 community monitoring; however, data on the stability of SARS-CoV-2 RNA in wastewater are needed to interpret WBE results, and MHV is suggested as suitable persistence surrogate.
Journal ArticleDOI
Detection of SARS-CoV-2 RNA in commercial passenger aircraft and cruise ship wastewater: a surveillance tool for assessing the presence of COVID-19 infected travellers.
Warish Ahmed,Paul M. Bertsch,Nicola Angel,Kyle Bibby,Aaron Bivins,Leanne Dierens,Janette Edson,John Ehret,Pradip Gyawali,Kerry A. Hamilton,Ian Hosegood,Philip Hugenholtz,Guangming Jiang,Masaaki Kitajima,Homa Teimouri Sichani,Jiahua Shi,Katja M. Shimko,Stuart L. Simpson,Wendy Smith,Erin M. Symonds,Kevin V. Thomas,Rory Verhagen,Julian Zaugg,Jochen F. Mueller +23 more
TL;DR: Surveillance of wastewater from large transport vessels with their own sanitation systems has potential as a complementary data source to prioritize clinical testing and contact tracing among disembarking passengers and must be further optimized to maximize detection sensitivity.
Journal ArticleDOI
Surveillance of SARS-CoV-2 RNA in wastewater: Methods optimisation and quality control are crucial for generating reliable public health information.
Warish Ahmed,Aaron Bivins,Paul M. Bertsch,Kyle Bibby,Phil M. Choi,Kata Farkas,Pradip Gyawali,Kerry A. Hamilton,Eiji Haramoto,Masaaki Kitajima,Stuart L. Simpson,Sarmila Tandukar,Kevin V. Thomas,Jochen F. Mueller +13 more
TL;DR: This review summarizes published studies of SARS-CoV-2 RNA detection in wastewater as well as available information regarding concentration, extraction, and detection methods and highlights areas for potential standardization.
Journal ArticleDOI
Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance
Warish Ahmed,Stuart L. Simpson,Paul M. Bertsch,Kyle Bibby,Aaron Bivins,Linda L. Blackall,Sílvia Bofill-Mas,Albert Bosch,João Brandão,Phil M. Choi,Phil M. Choi,Mark Ciesielski,Erica Donner,Nishita D'Souza,Andreas H. Farnleitner,Andreas H. Farnleitner,Daniel Gerrity,Raul Gonzalez,John F. Griffith,Pradip Gyawali,Charles N. Haas,Kerry A. Hamilton,Hapuarachchige Chanditha Hapuarachchi,Valerie J. Harwood,Rehnuma Haque,Greg Jackson,Stuart J. Khan,Wesaal Khan,Masaaki Kitajima,Asja Korajkic,Giuseppina La Rosa,Blythe A. Layton,Erin K. Lipp,Sandra L. McLellan,Brian R. McMinn,Gertjan Medema,Suzanne Metcalfe,Wim G. Meijer,Jochen F. Mueller,Heather M. Murphy,Coleen C. Naughton,Rachel T. Noble,Sudhi Payyappat,Susan Petterson,Tarja Pitkänen,Tarja Pitkänen,Verónica Beatriz Rajal,Brandon Reyneke,Fernando A. Roman,Joan B. Rose,Marta Rusiñol,Michael J. Sadowsky,Laura Sala-Comorera,Yin Xiang Setoh,Samendra P. Sherchan,Kwanrawee Sirikanchana,Wendy Smith,Joshua A. Steele,Rosalie Subburg,Erin M. Symonds,Phong K. Thai,Kevin V. Thomas,Josh Tynan,Simon Toze,Janelle R. Thompson,Andrew S. Whiteley,Judith Chui Ching Wong,Daisuke Sano,Stefan Wuertz,Stefan Wuertz,Irene Xagoraraki,Qian Zhang,Amity G. Zimmer-Faust,Orin C. Shanks +73 more
TL;DR: A technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors.