Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater.
Warish Ahmed,Paul M. Bertsch,Aaron Bivins,Kyle Bibby,Kata Farkas,Amy Gathercole,Eiji Haramoto,Pradip Gyawali,Asja Korajkic,Brian R. McMinn,Jochen F. Mueller,Stuart L. Simpson,Wendy Smith,Erin M. Symonds,Kevin V. Thomas,Rory Verhagen,Masaaki Kitajima +16 more
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TLDR
The results suggest that absorption-extraction methods with minimal pretreatment or without manipulation can provide suitably rapid, cost-effective and relatively straightforward recovery of enveloped viruses in wastewater.About:
This article is published in Science of The Total Environment.The article was published on 2020-10-15 and is currently open access. It has received 372 citations till now.read more
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SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed Cases
Fuqing Wu,Jianbo Zhang,Amy Xiao,Xiaoqiong Gu,Wei Lin Lee,Federica Armas,Kathryn M. Kauffman,William P. Hanage,Mariana Matus,Newsha Ghaeli,Noriko Endo,Claire Duvallet,Mathilde Poyet,Katya Moniz,Alex D. Washburne,Timothy B. Erickson,Timothy B. Erickson,Peter R. Chai,Peter R. Chai,Janelle R. Thompson,Eric J. Alm +20 more
TL;DR: A laboratory protocol to quantify viral titers in raw sewage via qPCR analysis and validate results with sequencing analysis suggests that the number of positive cases estimated from wastewater viral titer is orders of magnitude greater than the numberof confirmed clinical cases and therefore may significantly impact efforts to understand the case fatality rate and progression of disease.
Journal ArticleDOI
First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan.
TL;DR: Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community.
Journal ArticleDOI
First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA.
Samendra P. Sherchan,Shalina Shahin,Lauren M. Ward,Sarmila Tandukar,Tiong Gim Aw,Bradley W. Schmitz,Warish Ahmed,Masaaki Kitajima +7 more
TL;DR: To the knowledge, this is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in North America, including the USA, however, concentration methods and RT-qPCR assays need to be refined and validated to increase the sensitivity of Sars- CoV- 2 RNA detection in wastewater.
Journal ArticleDOI
First proof of the capability of wastewater surveillance for COVID-19 in India through detection of genetic material of SARS-CoV-2.
Manish Kumar,Arbind Kumar Patel,Anil V. Shah,Janvi Raval,Neha Rajpara,Madhvi Joshi,Chaitanya G. Joshi +6 more
TL;DR: The first ever successful effort in India to detect the genetic material of SARS-CoV-2 viruses to understand the capability and application of wastewater-based epidemiology (WBE) surveillance in India makes a strong case for the environmental surveillance of the COVID-19 pandemic.
Journal ArticleDOI
Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology.
Warish Ahmed,Paul M. Bertsch,Kyle Bibby,Eiji Haramoto,Joanne Hewitt,Flavia Huygens,Pradip Gyawali,Asja Korajkic,Shane Riddell,Samendra P. Sherchan,Stuart L. Simpson,Kwanrawee Sirikanchana,Erin M. Symonds,Rory Verhagen,Seshadri S. Vasan,Masaaki Kitajima,Aaron Bivins +16 more
TL;DR: Wastewater-based epidemiology (WBE) demonstrates potential for COVID-19 community monitoring; however, data on the stability of SARS-CoV-2 RNA in wastewater are needed to interpret WBE results, and MHV is suggested as suitable persistence surrogate.
References
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Journal ArticleDOI
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Stephen A. Bustin,Vladimir Benes,Jeremy A. Garson,Jan Hellemans,Jim F. Huggett,Mikael Kubista,Reinhold Mueller,Tania Nolan,Michael W. Pfaffl,Gregory L. Shipley,Jo Vandesompele,Carl T. Wittwer,Carl T. Wittwer +12 more
TL;DR: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency.
Journal ArticleDOI
The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2
Alexander E. Gorbalenya,Susan C. Baker,Ralph S. Baric,Raoul J. de Groot,Christian Drosten,Anastasia A. Gulyaeva,Bart L. Haagmans,Chris Lauber,Andrey M. Leontovich,Benjamin W. Neuman,Dmitry Penzar,Stanley Perlman,Leo L.M. Poon,Dmitry V. Samborskiy,Igor A. Sidorov,Isabel Sola,John Ziebuhr +16 more
TL;DR: The independent zoonotic transmission of SARS-CoV and SARS -CoV-2 highlights the need for studying viruses at the species level to complement research focused on individual pathogenic viruses of immediate significance.
Journal ArticleDOI
First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community.
Warish Ahmed,Nicola Angel,Janette Edson,Kyle Bibby,Aaron Bivins,Jake W. O'Brien,Phil M. Choi,Masaaki Kitajima,Stuart L. Simpson,Jiaying Li,Benjamin J. Tscharke,Rory Verhagen,Wendy Smith,Julian Zaugg,Leanne Dierens,Philip Hugenholtz,Kevin V. Thomas,Jochen F. Mueller +17 more
TL;DR: The quantification of SARS-CoV-2 in wastewater affords the ability to monitor the prevalence of infections among the population via wastewater-based epidemiology (WBE) and highlights the viability of WBE for monitoring infectious diseases, such as COVID-19, in communities.
Journal ArticleDOI
Airborne transmission of SARS-CoV-2: The world should face the reality.
Lidia Morawska,Junji Cao +1 more
TL;DR: It is extremely important, that the national authorities acknowledge the reality that the virus spreads through air, and recommend that adequate control measures be implemented to prevent further spread of the SARS-CoV-2 virus, in particularly removal of the virus-laden droplets from indoor air by ventilation.
Journal ArticleDOI
Presence of SARS-Coronavirus-2 RNA in Sewage and Correlation with Reported COVID-19 Prevalence in the Early Stage of the Epidemic in the Netherlands
TL;DR: The detection of the virus RNA in sewage, even when the CO VID-19 prevalence is low, and the correlation between concentration in sewage and reported prevalence of COVID-19, indicate that sewage surveillance could be a sensitive tool to monitor the circulation of theirus in the population.
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