Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance
Warish Ahmed,Stuart L. Simpson,Paul M. Bertsch,Kyle Bibby,Aaron Bivins,Linda L. Blackall,Sílvia Bofill-Mas,Albert Bosch,João Brandão,Phil M. Choi,Phil M. Choi,Mark Ciesielski,Erica Donner,Nishita D'Souza,Andreas H. Farnleitner,Andreas H. Farnleitner,Daniel Gerrity,Raul Gonzalez,John F. Griffith,Pradip Gyawali,Charles N. Haas,Kerry A. Hamilton,Hapuarachchige Chanditha Hapuarachchi,Valerie J. Harwood,Rehnuma Haque,Greg Jackson,Stuart J. Khan,Wesaal Khan,Masaaki Kitajima,Asja Korajkic,Giuseppina La Rosa,Blythe A. Layton,Erin K. Lipp,Sandra L. McLellan,Brian R. McMinn,Gertjan Medema,Suzanne Metcalfe,Wim G. Meijer,Jochen F. Mueller,Heather M. Murphy,Coleen C. Naughton,Rachel T. Noble,Sudhi Payyappat,Susan Petterson,Tarja Pitkänen,Tarja Pitkänen,Verónica Beatriz Rajal,Brandon Reyneke,Fernando A. Roman,Joan B. Rose,Marta Rusiñol,Michael J. Sadowsky,Laura Sala-Comorera,Yin Xiang Setoh,Samendra P. Sherchan,Kwanrawee Sirikanchana,Wendy Smith,Joshua A. Steele,Rosalie Subburg,Erin M. Symonds,Phong K. Thai,Kevin V. Thomas,Josh Tynan,Simon Toze,Janelle R. Thompson,Andrew S. Whiteley,Judith Chui Ching Wong,Daisuke Sano,Stefan Wuertz,Stefan Wuertz,Irene Xagoraraki,Qian Zhang,Amity G. Zimmer-Faust,Orin C. Shanks +73 more
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TLDR
A technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors.About:
This article is published in Science of The Total Environment.The article was published on 2022-01-20 and is currently open access. It has received 116 citations till now. The article focuses on the topics: Wastewater.read more
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Understanding and managing uncertainty and variability for wastewater monitoring beyond the pandemic: Lessons learned from the United Kingdom national COVID-19 surveillance programmes.
Matthew J. Wade,Bo Fritzbøger,Anna Lo Jacomo,Elena Armenise,Mathew R. Brown,Joshua T. Bunce,Graeme J Cameron,Zhou Fang,Kata Farkas,Deidre F Gilpin,David W. Graham,Jasmine M. S. Grimsley,Alwyn Hart,Till Hoffmann,Katherine J Jackson,David L. Jones,Chris J Lilley,John W. McGrath,Jennifer McKinley,Cormac McSparron,Behnam Firoozi Nejad,Mario Morvan,Marcos Quintela-Baluja,Adrian M I Roberts,Andrew C. Singer,Célia Souque,Vanessa Speight,Chris Sweetapple,David I. Walker,Glenn Watts,Andrew J. Weightman,Barbara Kasprzyk-Hordern +31 more
TL;DR: In this article, a discussion of measurement uncertainty associated with surveillance of wastewater, focusing on lessons-learned from the UK programmes monitoring COVID-19 is presented, showing that sources of uncertainty impacting measurement quality and interpretation of data for public health decision-making, are varied and complex.
Journal ArticleDOI
Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater
Warish Ahmed,Wendy Smith,Suzanne Metcalfe,Greg Jackson,Phil M. Choi,Mary Morrison,Daniel Field,Pradip Gyawali,Aaron Bivins,Kyle Bibby,Stuart L. Simpson +10 more
TL;DR: The positivity results indicated that for the analysis of SARS-CoV-2 RNA in wastewater, including the eluate and pellet samples may further increase the detection sensitivity using RT-dPCR.
Journal ArticleDOI
Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater
TL;DR: In this paper , the authors compared five PEG precipitation procedures, with different operational parameters, for the RT-qPCR-based whole process recovery efficiency of murine hepatitis virus (MHV), bacteriophage phi6, and pepper mild mottle virus (PMMoV) using 34 raw wastewater samples collected in Japan.
Journal ArticleDOI
Wastewater surveillance demonstrates high predictive value for COVID-19 infection on board repatriation flights to Australia
TL;DR: Wang et al. as mentioned in this paper showed that the surveillance of wastewater from aircraft for SARS-CoV-2 RNA can provide an additional and effective tool for informing the management of returning overseas travelers and for monitoring the importation of SARS CoV2 and other clinically significant pathogens.
References
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