Anhui Normal University

About: Anhui Normal University is a education organization based out in Wuhu, China. It is known for research contribution in the topics: Catalysis & Population. The organization has 7955 authors who have published 7309 publications receiving 117443 citations.

Colorimetric and Phosphorimetric Dual-Signaling Strategy Mediated by Inner Filter Effect for Highly Sensitive Assay of Organophosphorus Pesticides.

Abstract: We describe here a colorimetric and phosphorimetric dual-signaling strategy for sensitive assay of organophosphorus pesticides (OPPs). The principle for assay depends on the phenomenon that the phosphorescence of Mn-ZnS quantum dots (QDs) can be dramatically quenched by Au nanoparticles (AuNPs) through the inner filter effect (IFE) and the activity of acetylcholinesterase (AChE), an enzyme that catalytically hydrolyzes acetylthiocholine to thiocholine that can be inhibited by OPPs. By virtue of the variations of absorbance and phosphorescence of the analytical system, a dual-readout assay for OPPs has been proposed. The limits of detection for different OPPs including paraoxon, parathion, omethoate, and dimethyl dichlorovinyl phosphate (DDVP) are found to be 0.29, 0.59, 0.67, and 0.44 ng/L, respectively. The proposed assay was allowed to detect pesticides in real spiked samples and authentic contaminated apples with satisfactory results, suggesting its potential applications to detect pesticides in complicated samples.

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing.

Abstract: Transient expression of the CRISPR/Cas9 machinery will not only reduce risks of mutagenesis from off-target activities, but also decrease possible immune response to Cas9 protein. Building on our recent developing of a system able to package up to 100 copies of Cas9 mRNA in each lentivirus-like particle (LVLP) via the specific interaction between aptamer and aptamer-binding proteins (ABP), here we develop a lentiviral capsid-based bionanoparticle system, which allows efficient packaging of Cas9/sgRNA ribonucleoprotein (RNP). We show that replacing the Tetraloop of sgRNA scaffold with a com aptamer preserves the functions of the guide RNA, and the com-modified sgRNA can package Cas9/sgRNA RNP into lentivirus-like particles via the specific interactions between ABP and aptamer, and sgRNA and Cas9 protein. These RNP bionanoparticles generated Indels on different targets in different cells with efficiencies similar to or better than our recently described Cas9 mRNA LVLPs. The new system showed fast action and reduced off-target rates, and makes it more convenient and efficient in delivering Cas9 RNPs for transient Cas9 expression and efficient genome editing.

Concentrations and characteristics of selenium in soil samples from Dashan Region, a selenium-enriched area in China

Abstract: In order to clarify the total selenium (Se) content and species of Se in the soils of Dashan Region, a typical Se-enriched area in China, 69 soil samples with different land-use purposes were collected, and the concentrations of total Se, water-soluble Se, exchangeable Se, acid-soluble Se, organic-bound Se and residual Se and the physical and chemical properties of these soils were investigated. The total Se content ranged between 0.31 and 7.65 mg kg−1 (dry weight). The concentrations of water-soluble, exchangeable, acid-soluble, organic-bound and residual forms of Se were 7.71–26.8 μg kg−1, 24.7–93.3 μg kg−1, 112–1430 μg kg−1, 26–765 μg kg−1 and 1300–5330 μg kg−1, respectively. The concentrations of total Se were positively correlated with the contents of clay and soil organic matter, but were negatively correlated with pH values. The contents of bioavailable Se, which consists of water-soluble Se and exchangeable Se, were negatively correlated with the contents of soil organic matter, but positi...