Ghent University

About: Ghent University is a education organization based out in Ghent, Belgium. It is known for research contribution in the topics: Population & Context (language use). The organization has 36170 authors who have published 111042 publications receiving 3774501 citations. The organization is also known as: UGent & University of Ghent.

Addressing overreporting on the International Physical Activity Questionnaire (IPAQ) telephone survey with a population sample

Abstract: Objective: To examine a possible problem of overreporting and to describe the degree of error with the International Physical Activity Questionnaire (IPAQ) short telephone protocol. Design: Cross-sectional study, using two different physical activity (PA) self-report protocols. Setting: Telephone interviews about PA in Belgium. Subjects: Fifty adults who had previously been interviewed with IPAQ in a national survey. Results: Seventy-five per cent reported less PA with the modified procedure than with the IPAQ. Twenty-three of the 50 individuals were found to have reported some amounts of PA with the IPAQ (either walking, or vigorous or moderate PA) when they should have reported none. In total, based on their revised reports of PA, 50% fewer persons met PA recommendations than was the case with IPAQ. The overreporting could not be related to types of error-prone individuals. Conclusions: Overreporting of PA in population samples is a serious problem that could be reduced by implementing procedure changes without changing the IPAQ items themselves.

Dying for a cause: NETosis, mechanisms behind an antimicrobial cell death modality

Abstract: Neutrophil extracellular traps (NETs) are chromatin structures loaded with antimicrobial molecules. They can trap and kill various bacterial, fungal and protozoal pathogens, and their release is one of the first lines of defense against pathogens. In vivo, NETs are released during a form of pathogen-induced cell death, which was recently named NETosis. Ex vivo, both dead and viable neutrophils can be stimulated to release NETs composed of either nuclear or mitochondrial chromatin, respectively. In certain pathological conditions, NETs are associated with severe tissue damage or certain auto-immune diseases. This review describes the recent progress made in the identification of the mechanisms involved in NETosis and discusses its interplay with autophagy and apoptosis.

Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes.

Abstract: Rhizobia described so far belong to three distinct phylogenetic branches within the alpha-2 subclass of Proteobacteria. Here we report the discovery of a fourth rhizobial branch involving bacteria of the Methylobacterium genus. Rhizobia isolated from Crotalaria legumes were assigned to a new species, "Methylobacterium nodulans," within the Methylobacterium genus on the basis of 16S ribosomal DNA analyses. We demonstrated that these rhizobia facultatively grow on methanol, which is a characteristic of Methylobacterium spp. but a unique feature among rhizobia. Genes encoding two key enzymes of methylotrophy and nodulation, the mxaF gene, encoding the alpha subunit of the methanol dehydrogenase, and the nodA gene, encoding an acyltransferase involved in Nod factor biosynthesis, were sequenced for the type strain, ORS2060. Plant tests and nodA amplification assays showed that "M. nodulans" is the only nodulating Methylobacterium sp. identified so far. Phylogenetic sequence analysis showed that "M. nodulans" NodA is closely related to Bradyrhizobium NodA, suggesting that this gene was acquired by horizontal gene transfer.